High throughput analysis of 10 microsatellite and 11 diallelic polymorphisms on the human Y-chromosome

被引：79

作者：

Thomas, MG

Bradman, N

Flinn, HM

机构：

[1] UCL, Ctr Genet Anthropol, London WC1E 6BT, England

[2] UCL, Dept Anthropol, London WC1E 6BT, England

[3] UCL, Dept Biol, London WC1E 6BT, England

来源：

HUMAN GENETICS | 1999年 / 105卷 / 06期

关键词：

D O I：

10.1007/s004390051148

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We describe an integrated approach to the determination of complex Y chromosome haplotypes that is both fast and relatively inexpensive. The method employs GeneScan technology to enable a researcher to assay repeat number variation at ten microsatellite loci and determine the status of 11 diallelic polymorphisms. The method requires only four PCRs and four GeneScan runs per sample and is relatively insensitive to sample DNA concentration.

引用

页码：577 / 581

页数：5

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