Immobilisation of enzymes on poly(aniline)-poly(anion) composite films. Preparation of bioanodes for biofuel cell applications

被引：47

作者：

Simon, E

Halliwell, CM

Toh, CS

Cass, AEG

Bartlett, PN ^{[1
]}

机构：

[1] Univ Southampton, Dept Chem, Southampton SO17 1BJ, Hants, England

[2] Univ London Imperial Coll Sci Technol & Med, Dept Biochem, London SW7 2AY, England

来源：

BIOELECTROCHEMISTRY | 2002年 / 55卷 / 1-2期

关键词：

enzyme immobilisation; poly(aniline) composite films; NADH; biofuel cell;

D O I：

10.1016/S1567-5394(01)00160-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Immobilisation of enzymes is important for applications such as biosensors or biofuel cells. A poly(histidine) tag had been introduced on the C terminus of a lactate dehydrogenase enzyme. This mutant enzyme was then immobilised onto poly(aniline) (PANi)-poly(anion) composite films, PANi-poly(vinylsulfonate) (PVS) or PANi-poly(acrylate) (PAA). The NADH produced by the immobilised enzyme in the presence of beta-nicotinamide adenine dinucleotide (NAD+) and lactate is oxidised at the poly(aniline)-coated electrode at 0.05 to 0.1 V vs. saturated calomel electrode (SCE) at 35 degreesC. (C) 2002 Elsevier Science B.V All rights reserved.

引用

页码：13 / 15

页数：3

共 9 条

[1] Poly(aniline)-poly(acrylate) composite films as modified electrodes for the oxidation of NADH [J].