Electron microscopy of helical filaments: rediscovering buried treasures in negative stain

被引：4

作者：

Egelman, Edward H. ^{[1
]}

Amos, Linda A. ^{[2
]}

机构：

[1] Univ Virginia, Dept Biochem & Mol Genet, Charlottesville, VA 22908 USA

[2] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

来源：

BIOESSAYS | 2009年 / 31卷 / 09期

基金：

英国医学研究理事会;

关键词：

electron microscopy; MOSAIC VIRUS; MICROGRAPHS; COMPLEXES; PROTEINS;

D O I：

10.1002/bies.200900075

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Although negative stain electron microscopy is a wonderfully simple way of directly visualizing protein complexes and other biological macromolecules, the images are not really comparable to those of objects seen in everyday life. The failure to appreciate this has recently led to an incorrect interpretation of RecA-family filament structures.

引用

页码：909 / 911

页数：3

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