Evaluation of rapid DNA extraction methods for the quantitative detection of fungi using real-time PCR analysis

被引：147

作者：

Haugland, RA ^{[1
]}

Brinkman, N ^{[1
]}

Vesper, SJ ^{[1
]}

机构：

[1] US EPA, Natl Exposure Res Lab, Cincinnati, OH 45268 USA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2002年 / 50卷 / 03期

关键词：

DNA; extraction; fungi; real time; PCR; quantitative;

D O I：

10.1016/S0167-7012(02)00037-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Three comparatively rapid methods for the extraction of DNA from fungal conidia and yeast cells in environmental (air, water and dust) samples were evaluated for use in real-time PCR (TaqMan(TM)) analyses. A simple bead milling method was developed to provide sensitive, accurate and precise quantification of target organisms in air and water (tap and surface) samples. However, quantitative analysis of dust samples required further purification of the extracted DNA by a streamlined silica adsorption procedure. Published by Elsevier Science B.V.

引用

页码：319 / 323

页数：5

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