Effect of copper speciation on whole-cell soluble methane monooxygenase activity in Methylosinus trichosporium OB3b

被引：31

作者：

Morton, JD ^{[1
]}

Hayes, KF ^{[1
]}

Semrau, JD ^{[1
]}

机构：

[1] Univ Michigan, Dept Civil & Environm Engn, Ann Arbor, MI 48109 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 04期

关键词：

D O I：

10.1128/AEM.66.4.1730-1733.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Soluble methane monooxygenase (sMMO) activity in Methylosinus trichosporium OB3b was found to be more strongly affected as copper-to-biomass ratios changed in a newly developed medium, M2M, which uses pyrophosphate for metal ehelation, than in nitrate mineral salts (NMS), which uses EDTA When M2M medium was amended with EDTA sMMO activity was similar to that in NMS medium, indicating that EDTA-bound copper had lower bioavailability than pyrophosphate-bound copper. EDTA, did not limit the association of copper with the cells; rather, copper was sequestered in a form which did not affect sMMO activity.

引用

页码：1730 / 1733

页数：4

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