FISH studies identify the i(20q-) anomaly as a der(20)del(20)(q11q13)idic(20)(p11)

被引:16
作者
Li, TY [1 ]
Xue, YQ [1 ]
Wu, YF [1 ]
Pan, JL [1 ]
机构
[1] Jiangsu Inst Hematol, Affiliated Hosp Soochow 1, Suzhou 215006, Peoples R China
关键词
D O I
10.1002/gcc.20313
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Fluorescence in situ hybridization (FISH) analyses were performed on six of seven patients who had been reported in 2004 to have an i(20q-) anomaly expressed as ider(20)(q10)del(20)(q11q13). The i(20q-) was investigated with a series of probes: a centromere-specific probe for chromosome 20, two paint probes for 20p and 20q, and a panel of locus-specific probes prepared from BAC/PAC clones mapped to 20p. The results showed that: (1) i(20q-) was a dicentric chromosome; (2) both of its arms comprised a deleted 20q and a small part of 20p near the centromere of chromosome 20; and (3) the breakpoints and reunion sites of i(20q-) differed, residing in the region 20p11.21-20p11.22 delineated by BAC/PAC clones RP11-96L6 and RP13-401N8. Thus, i(20q-) could be more precisely described as a der(20)del(20)(q11q13)idic(20)(p11). (c) 2006 Wiley-Liss, Inc.
引用
收藏
页码:536 / 539
页数:4
相关论文
共 10 条
[1]  
Asimakopoulos FA, 1996, BRIT J HAEMATOL, V95, P219
[2]  
Cuneo A, 1999, TEXTBOOK OF MALIGNANT HAEMATOLOGY, P205
[3]  
DEWALD GW, 2003, CLIN LAB MED, P1
[4]   International scoring system for evaluating prognosis in myelodysplastic syndromes [J].
Greenberg, P ;
Cox, C ;
LeBeau, MM ;
Fenaux, P ;
Morel, P ;
Sanz, G ;
Sanz, M ;
Vallespi, T ;
Hamblin, T ;
Oscier, D ;
Ohyashiki, K ;
Toyama, K ;
Aul, C ;
Mufti, G ;
Bennett, J .
BLOOD, 1997, 89 (06) :2079-2088
[5]  
Hunger SP, 1999, TEXTBOOK OF MALIGNANT HAEMATOLOGY, P187
[6]  
ISCN, 1995, INT SYST HUM CYT NOM
[7]  
LEBEAU MM, 2001, WILLIAMS HEMATOLOGY, P115
[8]   Clinical and molecular cytogenetic studies in seven patients with myeloid diseases characterized by i(20q-) [J].
Li, TY ;
Xue, YQ ;
Wu, YF ;
Pan, JL .
BRITISH JOURNAL OF HAEMATOLOGY, 2004, 125 (03) :337-342
[9]   Isochromosome of a deleted 20q: a rare but recurrent chromosome abnormality in myelodysplastic syndromes [J].
Saunders, K ;
Czepulkowskia, B ;
Sivalingam, R ;
Hayes, JPLA ;
Aldouri, M ;
Sekhar, M ;
Cummins, M ;
Ho, A ;
Mufti, GJ .
CANCER GENETICS AND CYTOGENETICS, 2005, 156 (02) :154-157
[10]   DE-NOVO MYELODYSPLASTIC SYNDROME (MDS) WITH DELETION OF THE LONG ARM OF CHROMOSOME-20 - A SUBTYPE OF MDS WITH DISTINCT HEMATOLOGICAL AND PROGNOSTIC FEATURES [J].
WATTEL, E ;
LAI, JL ;
HEBBAR, M ;
PREUDHOMME, C ;
GRAHEK, D ;
MOREL, P ;
BAUTERS, F ;
FENAUX, P .
LEUKEMIA RESEARCH, 1993, 17 (11) :921-926