Production, characterization and gene cloning of the extracellular enzymes from the marine-derived yeasts and their potential applications

被引：66

作者：

Chi, Zhenming ^{[1
]}

Chi, Zhe ^{[1
]}

Zhang, Tong ^{[1
]}

Liu, Guanglei ^{[1
]}

Li, Jing ^{[1
]}

Wang, Xianghong ^{[1
]}

机构：

[1] Ocean Univ China, Key Lab Marine Genet & Gene Resource Exploitat, Minist Educ, Qingdao, Peoples R China

来源：

BIOTECHNOLOGY ADVANCES | 2009年 / 27卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Marine yeast; Cellulase; Alkaline protease; Aspartic protease; Amylase; Inulinase; Lipase; Killer toxin; Phytase; CRYPTOCOCCUS-AUREUS G7A; AUREOBASIDIUM-PULLULANS HN2-3; ACID PROTEASE GENE; ALKALINE PROTEASE; KILLER TOXIN; INULIN HYDROLYSIS; PICHIA-GUILLIERMONDII; SURFACE DISPLAY; PHYTASE PRODUCTION; MOLECULAR-CLONING;

D O I：

10.1016/j.biotechadv.2009.01.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this review article, the extracellular enzymes production, their properties and cloning of the genes encoding the enzymes from marine yeasts are overviewed. Several yeast strains which could produce different kinds of extracellular enzymes were selected from the culture collection of marine yeasts available in this laboratory. The strains selected belong to different genera such as Yarrowia, Aureobasidium, Pichia, Metschnikowia and Cryptococcus. The extracellular enzymes include cellulase, alkaline protease, aspartic protease, amylase, inulinase, lipase and phytase, as well as killer toxin. The conditions and media for the enzyme production by the marine yeasts have been optimized and the enzymes have been purified and characterized. Some genes encoding the extracellular enzymes from the marine yeast strains have been cloned, sequenced and expressed. It was found that some properties of the enzymes from the marine yeasts are unique compared to those of the homologous enzymes from terrestrial yeasts and the genes encoding the enzymes in marine yeasts are different from those in terrestrial yeasts. Therefore, it is of very importance to further study the enzymes and their genes from the marine yeasts. This is the first review on the extracellular enzymes and their genes from the marine yeasts. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：236 / 255

页数：20

共 177 条

[1] PURIFICATION AND CHARACTERIZATION OF A NEUTRAL PROTEASE FROM SACCHAROMYCOPSIS-LIPOLYTICA [J].