A simple and rapid determination of biapenem in plasma by high-performance liquid chromatography

被引：33

作者：

Ikeda, Kayo ^{[1
]}

Ikawa, Kazuro ^{[1
]}

Ikeda, Aki ^{[1
]}

Nishikawa, Yoshimi ^{[1
]}

Morikawa, Norifumi ^{[1
]}

机构：

[1] Hiroshima Univ, Dept Clin Pharmacol, Div Clin Pharmacotherapeut, Grad Sch Biomed Sci,Minami Ku, Hiroshima 7348553, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2006年 / 844卷 / 01期

关键词：

biapenem; ultratiltration;

D O I：

10.1016/j.jchromb.2006.06.023

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, rapid and precise HPLC method using ultrafiltration to remove plasma protein was developed to determine biapenem concentrations in human plasma. Plasma was separated by centrifugation at 4 degrees C from blood collected in heparinized vacuum tubes, and biapenem was stabilized by immediate mixing the plasma with 1 M 3-morpholinopropanesulfonic acid (MOPS) buffer (pH 7.0) (1: 1). Biapenem was detected by ultraviolet absorbance at 300 nm with no interfering plasma peak. The calibration curve of biapenem in human plasma was linear from 0.04 to 50 mu g/mL. The limit of detection was 0.01 mu g/mL, which was more than 40-fold lower than that of conventional plasma protein precipitation using ammonium sulfate. The assay has been clinically applied to pharmacokinetic studies in patients. (c) 2006 Elsevier B.V. All rights reserved.

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收藏

页码：148 / 152

页数：5

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