Regulation of proinflammatory cytokines in human lung epithelial cells infected with Mycoplasma pneumoniae

被引:113
作者
Yang, J
Hooper, WC
Phillips, DJ
Talkington, DF
机构
[1] CDCP, Div Bacterial & Mycot Dis, Natl Ctr Infect Dis, Atlanta, GA 30333 USA
[2] CDCP, Div Aids, Natl Ctr Infect Dis, Sexually Transmitted Dis & TB Lab, Atlanta, GA 30333 USA
关键词
D O I
10.1128/IAI.70.7.3649-3655.2002
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Mycoplasma pneumoniae is a small bacterium without a cell wall that causes tracheobronchitis and atypical pneumonia in humans. It has also been associated with chronic conditions, such as arthritis, and extrapulmonary complications, such as encephalitis. Although the interaction of mycoplasmas with respiratory epithelial cells is a critical early phase of pathogenesis, little is known about the cascade of events initiated by infection of respiratory epithelial cells by mycoplasmas. Previous studies have shown that M. pneumoniae can induce proinflammatory cytokines in several different study systems including cultured murine and human monocytes. In this study, we demonstrate that M. pneumoniae infection also induces proinflammatory cytokine expression in A549 human lung carcinoma cells. Infection of A549 cells resulted in increased levels of interleukin-8 (IL-8) and tumor necrosis factor alpha mRNA, and both proteins were secreted into culture medium. IL-1beta mRNA also increased after infection and IL-1beta protein was synthesized, but it remained intracellular. In contrast, levels of IL-6 and gamma interferon mRNA and protein remained unchanged or undetectable. Using protease digestion and antibody blocking methods, we found that M. pneumoniae cytadherence is important for the induction of cytokines. On the other hand, while M. pneumoniae protein synthesis and DNA synthesis do not appear to be prerequisites for the induction of cytokine gene expression, A549 cellular de novo protein synthesis is responsible for the increased cytokine protein levels. These results suggest a novel role for lung epithelia[ cells in the pathogenesis of M. pneumoniae infection and provide a better understanding of M. pneumoniae pathology at the cellular level.
引用
收藏
页码:3649 / 3655
页数:7
相关论文
共 54 条
[1]  
[Anonymous], MYCOPLASMAS MOL BIOL
[2]  
AURON PE, 1987, J IMMUNOL, V138, P1447
[3]   Mycoplasmas: Sophisticated, reemerging, and burdened by their notoriety [J].
Baseman, JB ;
Tully, JG .
EMERGING INFECTIOUS DISEASES, 1997, 3 (01) :21-32
[4]   INTERPLAY BETWEEN MYCOPLASMAS AND HOST TARGET-CELLS [J].
BASEMAN, JB ;
LANGE, M ;
CRISCIMAGNA, NL ;
GIRON, JA ;
THOMAS, CA .
MICROBIAL PATHOGENESIS, 1995, 19 (02) :105-116
[5]   Functional analysis of Mycoplasma arthritidis-derived mitogen interactions with class II molecules [J].
Bernatchez, C ;
AlDaccak, R ;
Mayer, PE ;
Mehindate, K ;
Rink, L ;
Mecheri, S ;
Mourad, W .
INFECTION AND IMMUNITY, 1997, 65 (06) :2000-2005
[6]   Gene transfer of a hybrid interleukin-1 beta gene to B16 mouse melanoma recruits leucocyte subsets and reduces tumour growth in vivo [J].
Bjorkdahl, O ;
Wingren, AG ;
Hedlund, G ;
Ohlsson, L ;
Dohlsten, M .
CANCER IMMUNOLOGY IMMUNOTHERAPY, 1997, 44 (05) :273-281
[7]   Role of interleukin-8 in community-acquired pneumonia: Relation to microbial load and pulmonary function [J].
Bohnet, S ;
Kotschau, U ;
Braun, J ;
Dalhoff, K .
INFECTION, 1997, 25 (02) :95-100
[8]   A 4TH-ORDER NONLINEAR ITERATIVE METHOD IN BANACH-SPACES [J].
CHEN, D ;
ARGYROS, IK .
APPLIED MATHEMATICS LETTERS, 1993, 6 (04) :97-98
[9]   IMPORTANCE OF ENDOGENOUS TUMOR-NECROSIS-FACTOR-ALPHA AND GAMMA-INTERFERON IN HOST-RESISTANCE AGAINST PNEUMOCYSTIS-CARINII INFECTION [J].
CHEN, WX ;
HAVELL, EA ;
HARMSEN, AG .
INFECTION AND IMMUNITY, 1992, 60 (04) :1279-1284
[10]   INTERLEUKIN-1 - AN IMPORTANT MEDIATOR OF HOST-RESISTANCE AGAINST PNEUMOCYTIS-CARINII [J].
CHEN, WX ;
HAVELL, EA ;
MOLDAWER, LL ;
MCINTYRE, KW ;
CHIZZONITE, RA ;
HARMSEN, AG .
JOURNAL OF EXPERIMENTAL MEDICINE, 1992, 176 (03) :713-718