Heterogeneous nuclear ribonucleoprotein E2 binds to tau exon 10 and moderately activates its splicing

被引：22

作者：

Broderick, J

Wang, JN

Andreadis, A ^{[1
]}

机构：

[1] UMMS, Shriver Ctr, Div Neurobiol, Waltham, MA 02452 USA

[2] Univ Massachusetts, Sch Med, Dept Cell Biol, Worcester, MA 01655 USA

来源：

GENE | 2004年 / 331卷

关键词：

regulated isoforms; alternative splicing regulators; frontotemporal dementia;

D O I：

10.1016/j.gene.2004.02.005

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Tau is a microtubule-associated protein whose transcript undergoes complex-regulated splicing in the mammalian nervous system. Exon 10 of the gene is an alternatively spliced cassette, which is adult-specific and codes for a microtubule-binding domain. Mutations that affect splicing of exon 10 have been shown to cause frontotemporal dementia with parkinsonism (FTDP). Using tau exon 10 as a bait in a yeast three-hybrid screen, we discovered that it interacts with hnRNPE2. Cotransfection assays show that hnRNPE2 isoforms moderately activate the splicing of exon 10. (C) 2004 Elsevier B.V. All rights reserved.

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页码：107 / 114

页数：8

相关论文

共 40 条

[1] STRUCTURE AND NOVEL EXONS OF THE HUMAN-TAU GENE [J].

ANDREADIS, A ;

BROWN, WM ;

KOSIK, KS .

BIOCHEMISTRY, 1992, 31 (43) :10626-10633

[2] Mechanisms of alternative pre-messenger RNA splicing [J].

Black, DL .

ANNUAL REVIEW OF BIOCHEMISTRY, 2003, 72 :291-336

[3]

Black MM, 1996, J NEUROSCI, V16, P3601

[4] INTERACTION OF TAU WITH THE NEURAL PLASMA-MEMBRANE MEDIATED BY TAU AMINO-TERMINAL PROJECTION DOMAIN [J].

BRANDT, R ;

LEGER, J ;

LEE, G .

JOURNAL OF CELL BIOLOGY, 1995, 131 (05) :1327-1340

[5]

Buckanovich RJ, 1996, J NEUROSCI, V16, P1114

[6]

Chabot B, 2003, Prog Mol Subcell Biol, V31, P59

[7] A novel set of nuclear localization signals determine distributions of the αCP RNA-binding proteins [J].

Chkheidze, AN ;

Liebhaber, SA .

MOLECULAR AND CELLULAR BIOLOGY, 2003, 23 (23) :8405-8415

[8] Determinants of 4-repeat tau expression - coordination between enhancing and inhibitory splicing sequences for exon 10 inclusion [J].

D'Souza, I ;

Schellenberg, GD .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (23) :17700-17709

[9] Tau exon 10 expression involves a bipartite intron 10 regulatory sequence and weak 5′ and 3′ splice sites. [J].

D'Souza, I ;

Schellenberg, GD .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (29) :26587-26599

[10] Missense and silent tau gene mutations cause frontotemporal dementia with parkinsonism-chromosome 17 type, by affecting multiple alternative RNA splicing regulatory elements [J].

D'Souza, I ;

Poorkaj, P ;

Hong, M ;

Nochlin, D ;

Lee, VMY ;

Bird, TD ;

Schellenberg, GD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (10) :5598-5603

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