Altered miRNA expression profiles are involved in the protective effects of troxerutin against ultraviolet B radiation in normal human dermal fibroblasts

被引：13

作者：

Cha, Hwa Jun ^{[1
,2
]}

Lee, Kwang Sik ^{[3
,4
,5
]}

Lee, Ghang Tai ^{[3
,4
,5
]}

Lee, Kun Kook ^{[3
]}

Hong, Jin Tae ^{[4
,5
]}

Lee, Sung Nae ^{[6
]}

Jang, Hyun Hee ^{[7
]}

Lee, Jae Ho ^{[8
,9
]}

Park, In-Chul ^{[10
]}

Kim, Yu Ri ^{[11
]}

Ahn, Kyu Joong ^{[11
]}

Kwon, Seung Bin ^{[1
,2
]}

An, In-Sook ^{[1
,2
]}

An, Sungkwan ^{[1
,2
]}

Bae, Seunghee ^{[1
,2
]}

机构：

[1] Konkuk Univ, Korea Inst Skin & Clin Sci, Seoul 143701, South Korea

[2] Konkuk Univ, Mol Targeted Drug Res Ctr, Seoul 143701, South Korea

[3] Coreana Cosmet Co Ltd, Songpa R&D Ctr, Cheonan 330833, Chungcheongnam, South Korea

[4] Chungbuk Natl Univ, Coll Pharm, Cheongju 361763, Chungcheongbuk, South Korea

[5] Chungbuk Natl Univ, Med Res Ctr, Cheongju 361763, Chungcheongbuk, South Korea

[6] Kyung In Womens Coll, Dept Cosmetol, Inchon 407740, South Korea

[7] Kyungbok Univ, Sch Art, Namyangju 472948, Gyeonggi DO, South Korea

[8] Kwandong Univ, Coll Med, Mol Oncol Lab, Cheil Gen Hosp, Seoul 100380, South Korea

[9] Kwandong Univ, Coll Med, Womens Healthcare Ctr, Seoul 100380, South Korea

[10] Korea Inst Radiol & Med Sci, Lab Funct Genom, Seoul 139706, South Korea

[11] Konkuk Univ, Sch Med, Dept Dermatol, Seoul 143701, South Korea

来源：

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2014年 / 33卷 / 04期

关键词：

troxerutin; ultraviolet B; normal human dermal fibroblasts; cell death; microRNA; EXTRACELLULAR-MATRIX; UVB IRRADIATION; DNA-REPAIR; HUMAN SKIN; PROTEIN; MICE; KERATINOCYTES; INFLAMMATION; SCLERODERMA; INHIBITION;

D O I：

10.3892/ijmm.2014.1647

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

The aim of this study was to investigate the mechanisms by which troxerutin protects cells against ultraviolet B (UVB) radiation. First, we demonstrate that pre-treatment with troxerutin protects normal human dermal fibroblasts (nHDFs) against UVB-induced cytotoxicity. As shown by migration assay and DNA repair analysis, troxerutin increased cell migration and DNA repair activity in the nHDFs. Subsequently, we analyzed microRNA (miRNA) expression profiles in the nHDFs. miRNAs are 19- to 24-nucleotide (nt) non-coding RNA molecules that regulate the translation of target genes through RNA interference. In UVB-exposed cells, miRNAs act on crucial functions, such as apoptosis and cellular senescence. miRNA expression is significantly altered during the protective process induced by phytochemicals. Therefore, understanding changes that occur in miRNA expression profiles may help to elucidate the protective mechanisms of troxerutin. We identified 11 miRNAs that were significantly (>2-fold) upregulated and 12 that were significantly downregulated (>2-fold) following treatment of the nHDFs with troxerutin. In addition, we investigated the biological functions of these miRNAs through the prediction of miRNA targets and Gene Ontology analysis of the putative targets. Overall, our findings indicate that pre-treatment with troxerutin increases the viability of UVB-exposed nHDFs through the alteration of the miRNA expression profiles.

引用

页码：957 / 963

页数：7

共 35 条

[1]

Involvement of microRNAs in epigallocatechin gallate-mediated UVB protection in human dermal fibroblasts [J].