Comparison of HLA-A antigen typing by serology with two polymerase chain reaction based DNA typing methods: Implications for proficiency testing

被引:25
作者
Bozon, MV
Delgado, JC
Turbay, D
Salazar, M
Granja, CB
Alosco, SM
Dupont, B
Yunis, EJ
机构
[1] AMER RED CROSS NEW ENGLAND REG,DEDHAM,MA
[2] SLOAN KETTERING INST CANC RES,NEW YORK,NY
来源
TISSUE ANTIGENS | 1996年 / 47卷 / 06期
关键词
HLA typing; sequence-specific oligonucleotide primer; sequence-specific primer; serology; proficiency testing;
D O I
10.1111/j.1399-0039.1996.tb02593.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Serology has been routinely used for class I HLA typing for the selection of donors for allotransplantation. However, serology is not adequate for the assignment of all class I specificities especially when testing non-Caucasians subjects and it is necessary to adopt new strategies for routine testing. At the present time the extent of incorrect serologic HLA-A assignments in clinical testing is nor known. The polymerase chain reaction (PCR) based techniques have become useful standard clinical typing methods of HLA class II alleles but most laboratories still use serology for class I typing. In this report we have compared two PCR based techniques, PCR amplification with sequence-specific primers (PCR-SSP) and PCR amplification and subsequent hybridization with sequence-specific oligonucleotide probes (PCR-SSOP), for the assignment of HLA-A specificities in 56 blood samples from patients and families serologically typed for HLA-A. This side-by-side comparison of PCR methods showed 100% correlation between them. However, serology showed 7.1% misassignments and, in an additional panel of 19 cells where serology produced equivocal results, the PCR-SSP and SSOP methods identified the correct HLA-A specificity. Our results emphasize the need to complement routine serologic testing of HLA specificities with a small number of primers designed to test HLA-A34, A36, A43, A66, A74 and A80, that are not detected with high precision by serology. We concluded that the PCR-SSP and -SSOP methods can be used in routine HLA-A typing of patients and donors for transplantation with a greater precision than serology.
引用
收藏
页码:512 / 518
页数:7
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