Identification of α-fodrin as an autoantigen in experimental coronavirus retinopathy (ECOR)

被引：24

作者：

Chin, Marian S. ^{[1
]}

Hooper, Laura C. ^{[1
]}

Hooks, John J. ^{[1
]}

Detrick, Barbara ^{[2
]}

机构：

[1] NEI, Immunol Lab, Immunol & Virol Sect, NIH, Bethesda, MD 20892 USA

[2] Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD 21205 USA

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2014年 / 272卷 / 1-2期

关键词：

Coronavirus; Retinal degeneration; alpha-Fodrin; Autoantibodies; Autoimmunity; CANCER-ASSOCIATED RETINOPATHY; PIGMENT EPITHELIAL-CELL; AUTOIMMUNE RETINOPATHY; ANTIRETINAL ANTIBODIES; RETINAL DEGENERATION; ALZHEIMERS-DISEASE; BRAIN SPECTRIN; ANTI-RECOVERIN; T-CELLS; IN-VIVO;

D O I：

10.1016/j.jneuroim.2014.05.002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

The coronavirus, mouse hepatitis virus (MHV), JHM strain induces a biphasic disease in BALB/c mice that consists of an acute retinitis followed by progression to a chronic retinal degeneration with autoimmune reactivity. Retinal degeneration resistant CD-1 mice do not develop either the late phase or autoimmune reactivity. A mouse RPE/choroid DNA expression library was screened using sera from virus infected BALB/c mice. Two clones were identified, villin-2 protein and alpha-fodrin protein. alpha-Fodrin protein was used for further analysis and western blot reactivity was seen only in sera from virus infected BALB/c mice. CD4 T cells were shown to specifically react with MHV antigens and with alpha-fodrin protein. These studies clearly identified both antibody and CD4 T cell reactivities to alpha-fodrin in sera from virus infected, retinal degenerative susceptible BALB/c mice. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：42 / 50

页数：9

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