Detection of both hepatitis A virus and Norwalk-like virus in imported clams associated with food-borne illness

被引:58
作者
Kingsley, DH [1 ]
Meade, GK [1 ]
Richards, GP [1 ]
机构
[1] Delaware State Univ, USDA ARS, Microbial Food Safety Res Unit, WW Baker Ctr, Dover, DE 19901 USA
关键词
D O I
10.1128/AEM.68.8.3914-3918.2002
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Hepatitis A virus (HAV) and Norwalk-like virus (NLV) were detected by reverse transcription-PCR in clams imported into the United States from China. An epidemiological investigation showed that these clams were associated with five cases of Norwalk-like gastroenteritis in New York State in August 2000 (Food and Drug Administration Import Alert 16-50). They were labeled "cooked" but appeared raw. Viral RNA extraction was performed by using dissected digestive tissues rather than whole shellfish meats, this was followed by glycine buffer elution, polyethylene glycol precipitation, Tri-Reagent treatment, and purification of poly(A) RNA with magnetic beads coupled to poly(dT) oligonucleotides. We identified HAV and NLV as genotype I and genogroup II strains, respectively. Both viruses have high levels of homology to Asian strains. An analysis of fecal coliforms revealed a most-probable number of 93,000/100 g of clam meat, which is approximately 300-fold higher than the hygienic standard for shellfish meats.
引用
收藏
页码:3914 / 3918
页数:5
相关论文
共 32 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]   Genetic classification of "Norwalk-like viruses" [J].
Ando, T ;
Noel, JS ;
Fankhauser, RL .
JOURNAL OF INFECTIOUS DISEASES, 2000, 181 :S336-S348
[3]   IMMUNE-RESPONSE AND PREVALENCE OF ANTIBODY TO NORWALK ENTERITIS VIRUS AS DETERMINED BY RADIOIMMUNOASSAY [J].
BLACKLOW, NR ;
CUKOR, G ;
BEDIGIAN, MK ;
ECHEVERRIA, P ;
GREENBERG, HB ;
SCHREIBER, DS ;
TRIER, JS .
JOURNAL OF CLINICAL MICROBIOLOGY, 1979, 10 (06) :903-909
[4]   COMPLETE NUCLEOTIDE-SEQUENCE OF WILD-TYPE HEPATITIS-A VIRUS - COMPARISON WITH DIFFERENT STRAINS OF HEPATITIS-A VIRUS AND OTHER PICORNAVIRUSES [J].
COHEN, JI ;
TICEHURST, JR ;
PURCELL, RH ;
BUCKLERWHITE, A ;
BAROUDY, BM .
JOURNAL OF VIROLOGY, 1987, 61 (01) :50-59
[5]   Genetic relatedness of Cuban HAV wild-type isolates [J].
Diaz, BI ;
Sariol, CA ;
Normann, A ;
Rodríguez, L ;
Flehmig, B .
JOURNAL OF MEDICAL VIROLOGY, 2001, 64 (02) :96-103
[6]   DETECTION AND OCCURRENCE OF ENTERIC VIRUSES IN SHELLFISH - REVIEW [J].
GERBA, CP ;
GOYAL, SM .
JOURNAL OF FOOD PROTECTION, 1978, 41 (09) :743-754
[7]   A polymerase chain reaction-based method for the detection of hepatitis A virus in produce and shellfish [J].
Goswami, BB ;
Kulka, I ;
Ngo, D ;
Istafanos, P ;
Cebula, TA .
JOURNAL OF FOOD PROTECTION, 2002, 65 (02) :393-402
[8]   AN EPIDEMIC OF HEPATITIS-A ATTRIBUTABLE TO THE INGESTION OF RAW CLAMS IN SHANGHAI, CHINA [J].
HALLIDAY, ML ;
KANG, LY ;
ZHOU, TK ;
HU, MD ;
PAN, QC ;
FU, TY ;
HUANG, YS ;
HU, SL .
JOURNAL OF INFECTIOUS DISEASES, 1991, 164 (05) :852-859
[9]   Evaluation of nine sets of PCR primers in the RNA dependent RNA polymerase region for detection and differentiation of members of the family Caliciviridae, Norwalk virus and Sapporo virus [J].
Honma, S ;
Nakata, S ;
Kinoshita-Numata, K ;
Kogawa, K ;
Chiba, S .
MICROBIOLOGY AND IMMUNOLOGY, 2000, 44 (05) :411-419
[10]   A multistate, foodborne outbreak of hepatitis A [J].
Hutin, YJF ;
Pool, V ;
Cramer, EH ;
Nainan, OV ;
Weth, J ;
Williams, IT ;
Goldstein, ST ;
Gensheimer, KF ;
Bell, BP ;
Shapiro, CN ;
Alter, MJ ;
Margolis, HS .
NEW ENGLAND JOURNAL OF MEDICINE, 1999, 340 (08) :595-602