Expression and clinical significance of the long non-coding RNA PVT1 in human gastric cancer

被引：116

作者：

Ding, Jian ^{[1
]}

Li, Dan ^{[2
]}

Gong, Minzhen ^{[1
]}

Wang, Jinpo ^{[1
]}

Huang, Xunru ^{[1
]}

Wu, Ting ^{[1
]}

Wang, Chengdang ^{[1
]}

机构：

[1] Fujian Med Univ, Affiliated Hosp 1, Digest Dept, Fuzhou, Fujian, Peoples R China

[2] Fujian Med Univ, Union Hosp, Digest Dept, Fuzhou, Fujian, Peoples R China

来源：

ONCOTARGETS AND THERAPY | 2014年 / 7卷

基金：

美国国家科学基金会;

关键词：

microarray analysis; quantitative polymerase chain reaction; lymph node invasion; tumor biomarkers; paclitaxel resistance; IDENTIFICATION; TRANSLOCATIONS; MICRORNAS; GENOME; MYC; TRANSCRIPTION; METASTASIS; LYMPHOMA; 8Q24;

D O I：

10.2147/OTT.S68854

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Background: Highly sensitive markers are urgently needed for the diagnosis and grading of gastric cancer and for managing drug resistance. The recent identification of long-non-coding RNAs (lncRNAs) has provided new approaches for resolving this challenge. The aim of this study was to screen and identify new biomarkers for human gastric cancer from lncRNAs. Methods: First, we used lncRNA microarrays to conduct a preliminary screening for candidate lncRNAs of gastric cancer biomarkers in both human gastric cancer tissues and in two gastric cancer cell lines, SGC7901 cells and paclitaxel-resistant SGC7901 cells. The lncRNA plasmacytoma variant translocation 1 (PVT1) was found to exhibit higher expression in both gastric cancer tissues and the SGC7901 paclitaxel-resistant cell line. Quantitative polymerase chain reaction was used for large-scale analysis in a large number of human gastric cancer tissues to verify the involvement of PVT1 in development of gastric cancer. The relationships between PVT1 expression and clinical features were also analyzed. Results: PVT1 showed higher expression in human gastric cancer tissues than in adjacent non-cancerous tissues and in SGC7901 paclitaxel-resistant cells compared with SGC7901 cells. PVT1 expression was correlated with lymph node invasion of gastric cancer. Conclusion: PVT1 is a new biomarker for human gastric cancer and may indicate lymph node invasion. Therefore, PVT1 shows potential as a novel therapeutic target for the treatment of gastric cancer and enhancement of paclitaxel sensitivity.

引用

页码：1625 / 1630

页数：6

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