Generation of DNA Interstrand Cross-Links by Post-Synthetic Reductive Amination

被引：62

作者：

Angelov, Todor ^{[1
]}

Guainazzi, Angelo ^{[2
,3
]}

Schaerer, Orlando D. ^{[1
,2
,3
]}

机构：

[1] Univ Zurich, Inst Mol Canc Res, CH-8057 Zurich, Switzerland

[2] SUNY Stony Brook, Dept Pharmacol Sci, Stony Brook, NY 11794 USA

[3] SUNY Stony Brook, Dept Chem, Stony Brook, NY 11794 USA

来源：

ORGANIC LETTERS | 2009年 / 11卷 / 03期

基金：

瑞士国家科学基金会;

关键词：

STRUCTURAL-CHARACTERIZATION; DEOXYGUANOSINE RESIDUES; OLIGONUCLEOTIDE; DUPLEXES; MODEL;

D O I：

10.1021/ol802719a

中图分类号：

O62 [有机化学];

学科分类号：

070303 [有机化学];

摘要：

DNA interstrand cross-links (ICLs) are the clinically most relevant adducts formed by many antitumor agents. To facilitate the study of biological responses triggered by ICLs, we developed a new approach toward the synthesis of mimics of nitrogen mustard ICLs. 7-Deazaguanine residues bearing acetaldehyde groups were incorporated into complementary strands of DNA and cross-link formation induced by double reductive amination. Our strategy enables the synthesis of major groove cross-links in high yields and purity.

引用

页码：661 / 664

页数：4

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