Differential screening identifies genetic markers of monocyte to macrophage maturation

被引:165
作者
Krause, SW
Rehli, M
Kreutz, M
Schwarzfischer, L
Paulauskis, JD
Andreesen, R
机构
[1] UNIV REGENSBURG, DEPT INTERNAL MED, D-8400 REGENSBURG, GERMANY
[2] HARVARD UNIV, SCH PUBL HLTH, PHYSIOL PROGRAM, BOSTON, MA 02115 USA
关键词
cell differentiation; gene expression; library construction; DNA hybridization;
D O I
10.1002/jlb.60.4.540
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Maturation of cells of the mononuclear phagocyte lineage from bone marrow precursors to tissue macrophages (MAC) via circulating blood monocytes (MO) is a multistep process only partially understood, Similarly, MAC differentiation can be observed if MO are cultured in vitro. In an attempt to further characterize molecular changes occurring during this process we carried out differential screening of a MO-derived MAC cDNA library using MO and MAC cDNA, After subcloning and confirmation by a second round of screening, partial sequencing of 41 cDNA clones was performed, In 33 clones the sequences of 7 different previously identified cDNAs were found, The mRNA expression of two of the corresponding genes (apolipoprotein E, ferritin light chain) is already known to be up-regulated during MAC maturation. For one gene (cathepsin B), a specific up-regulation of mRNA expression could be shown corresponding to previous protein data, For four genes [human cartilage glycoprotein (HC-gp39), osteopontin, type IV collagenase, and tryptophanyl-tRNA synthetase] the specific expression in MAC versus MO was previously unknown but could be confirmed by the use of Northern blot analysis, Of these genes, HC-gp39 is especially interesting because it is only expressed during the late stages of MAC differentiation.
引用
收藏
页码:540 / 545
页数:6
相关论文
共 39 条
[1]  
ANDREESEN R, 1986, BLOOD, V67, P1257
[2]   SURFACE PHENOTYPE ANALYSIS OF HUMAN MONOCYTE TO MACROPHAGE MATURATION [J].
ANDREESEN, R ;
BRUGGER, W ;
SCHEIBENBOGEN, C ;
KREUTZ, M ;
LESER, HG ;
REHM, A ;
LOHR, GW .
JOURNAL OF LEUKOCYTE BIOLOGY, 1990, 47 (06) :490-497
[3]   PRIMARY CULTURES OF HUMAN BLOOD-BORNE MACROPHAGES GROWN ON HYDROPHOBIC TEFLON MEMBRANES [J].
ANDREESEN, R ;
PICHT, J ;
LOHR, GW .
JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 56 (03) :295-304
[4]  
ANDREESEN R, 1983, CANCER RES, V43, P5931
[5]  
ANDREESEN R, 1989, BLOOD, V74, P2150
[6]  
BECKER S, 1984, J IMMUNOL, V132, P1249
[7]  
BROWN LF, 1994, AM J PATHOL, V145, P610
[8]   MACROPHAGE COLONY-STIMULATING FACTOR IS REQUIRED FOR HUMAN MONOCYTE SURVIVAL AND ACTS AS A COFACTOR FOR THEIR TERMINAL DIFFERENTIATION TO MACROPHAGES INVITRO [J].
BRUGGER, W ;
KREUTZ, M ;
ANDREESEN, R .
JOURNAL OF LEUKOCYTE BIOLOGY, 1991, 49 (05) :483-488
[9]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[10]   OSTEOPONTIN - A PROTEIN WITH DIVERSE FUNCTIONS [J].
DENHARDT, DT ;
GUO, XJ .
FASEB JOURNAL, 1993, 7 (15) :1475-1482