Lipoxygenase inhibitory activity of octyl gallate

被引：36

作者：

Ha, TJ ^{[1
]}

Nihei, KI ^{[1
]}

Kubo, I ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2004年 / 52卷 / 10期

关键词：

soybean lipoxygenase; octyl gallate; competitive inhibitor; Lineweaver-Burk plots; antioxidant;

D O I：

10.1021/jf034925k

中图分类号：

S [农业科学];

学科分类号：

09 [农学];

摘要：

Octyl gallate inhibited soybean lipoxygenase-1 (EC 1.13.11.12, type 1) with an IC50 of 1.3 muM. The inhibition of the enzyme by octyl gallate is a slow and reversible reaction without residual activity. The inhibition kinetics analyzed by Lineweaver-Burk plots indicates that octyl gallate is a competitive inhibitor, and the inhibition constant, K-I, was obtained as 0.54 muM. One molecule of octyl gallate scavenged six molecules of 1,1-diphenyl-2-picrylhydrazyl and inhibited autoxidative lipid peroxidation. In addition, octyl gallate was effective in preventing lipid peroxidation.

引用

页码：3177 / 3181

页数：5

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