TLR4-mediated expulsion of bacteria from infected bladder epithelial cells

被引：116

作者：

Song, Jeongmin ^{[1
]}

Bishop, Brian L. ^{[1
]}

Li, Guojie ^{[2
]}

Grady, Richard ^{[5
]}

Stapleton, Ann ^{[6
,7
]}

Abraham, Soman N. ^{[1
,2
,3
,4
]}

机构：

[1] Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA

[2] Duke Univ, Med Ctr, Dept Pathol, Durham, NC 27710 USA

[3] Duke Univ, Med Ctr, Dept Immunol, Durham, NC 27710 USA

[4] Duke Natl Univ Singapore, Grad Sch Med, Program Emerging Infect Dis, Singapore 169547, Singapore

[5] Univ Washington, Dept Urol, Seattle, WA 98195 USA

[6] Univ Washington, Dept Med, Seattle, WA 98195 USA

[7] Univ Washington, Div Allergy & Infect Dis, Seattle, WA 98195 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 35期

基金：

美国国家卫生研究院;

关键词：

caveolin; exocytosis; signaling; urinary tract infection; uropathogenic Escherichia coli; URINARY-TRACT-INFECTION; ESCHERICHIA-COLI; TYPE-1; FIMBRIAE; PROTEIN; INVASION; PHOSPHORYLATION; LOCALIZATION; RECOGNITION; EXOCYTOSIS; PROTECTION;

D O I：

10.1073/pnas.0900527106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Uropathogenic Escherichia coli invade bladder epithelial cells (BECs) by direct entry into specialized cAMP regulated exocytic compartments. Remarkably, a significant number of these intracellular bacteria are subsequently expelled in a nonlytic and piecemeal fashion by infected BECs. Here, we report that expulsion of intracellular E. coli by infected BECs is initiated by the pattern recognition receptor, Toll-like receptor (TLR) 4, after activation by LPS. Also, we reveal that caveolin-1, Rab27b, PKA, and MyRIP are components of the exocytic compartment, and that they form a complex involved in the exocytosis of bacteria. This capacity of TLR4 to mediate the expulsion of intracellular bacteria from infected cells represents a previously unrecognized function for this innate immune receptor.

引用

页码：14966 / 14971

页数：6

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