Chromatin Binding of SRp20 and ASF/SF2 and Dissociation from Mitotic Chromosomes Is Modulated by Histone H3 Serine 10 Phosphorylation

被引：146

作者：

Loomis, Rebecca J. ^{[1
,2
]}

Naoe, Yoshinori ^{[1
,2
]}

Parker, J. Brandon ^{[1
,2
,3
]}

Savic, Velibor ^{[3
]}

Bozovsky, Matthew R. ^{[1
,2
]}

Macfarlan, Todd ^{[3
]}

Manley, James L. ^{[4
]}

Chakravarti, Debabrata ^{[1
,2
]}

机构：

[1] Northwestern Univ, Feinberg Sch Med, Div Reprod Biol Res, Dept Obstet & Gynecol, Chicago, IL 60611 USA

[2] Northwestern Univ, Feinberg Sch Med, Robert H Lurie Comprehens Canc Ctr, Chicago, IL 60611 USA

[3] Univ Penn, Grad Program Biol Sci, Philadelphia, PA 19104 USA

[4] Columbia Univ, Dept Biol Sci, New York, NY 10027 USA

来源：

MOLECULAR CELL | 2009年 / 33卷 / 04期

关键词：

SPLICING FACTOR ASF/SF2; SR PROTEINS; FISSION YEAST; CELL-CYCLE; LYSINE; 9; HETEROCHROMATIN; HP1; CONDENSATION; KINASE; RECOGNITION;

D O I：

10.1016/j.molcel.2009.02.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Histone H3 serine 10 phosphorylation is a hallmark of mitotic chromosomes, but its full function remains to be elucidated. We report here that two SR protein splicing factors, SRp20 and ASF/SF2, associate with interphase chromatin, are released from hyper-phosphorylated mitotic chromosomes, but reassociate with chromatin late in M-phase. Inhibition of Aurora B kinase diminished histone H3 serine 10 phosphorylation and increased SRp20 and ASH SF2 retention on mitotic chromosomes. Unexpectedly, we also found that HP1 proteins interact with ASF/SF2 in mitotic cells. Strikingly, siRNA-mediated knockdown of ASF/SF2 caused retention of HP1 proteins on mitotic chromatin. Finally, ASF/SF2-depleted cells released from a mitotic block displayed delayed G0/G1 entry, suggesting a functional consequence of these interactions. These findings underscore the evolving role of histone H3 phosphorylation and demonstrate a direct, functional, and histone-modification-regulated association of SRp20 and ASF/SF2 with chromatin.

引用

页码：450 / 461

页数：12

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