Preparative Separation of Axifolin-3-Glucoside, Hyperoside and Amygdalin from Plant Extracts by High Speed Countercurrent Chromatography

被引：22

作者：

Wei, Yun ^{[1
]}

Xie, Qianqian ^{[1
]}

Ito, Yoichiro ^{[2
]}

机构：

[1] Beijing Univ Chem Technol, Fac Sci, Dept Analyt Chem, Beijing 100029, Peoples R China

[2] NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2009年 / 32卷 / 07期

关键词：

Agrimonia pilosa Ledeb; Amygdalin; High speed countercurrent chromatography (HSCCC); Hyperoside; Taxifolin-3-glucoside;

D O I：

10.1080/10826070902790983

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

High speed countercurrent chromatography (HSCCC) was successfully used to isolate three bioactive compounds, i.e., amygdalin from bitter almond and taxifolin-3-glucoside and quercetin-3-galactoside (hyperoside) from water extract of A. pilosa Ledeb, respectively. From 1g of the crude extract 65mg of amygdalin was isolated at 97% purity using a two phase solvent system composed of ethyl acetate-n-butanol-water (5:2:5, v/v) by preparative HSCCC. From a 400mg amount of crude extract of A. pilosa Ledeb, 11mg of taxifolin-3-glucoside and 8mg of hyperoside were isolated at 96% purity, using a two phase solvent system composed of ethyl acetate-methanol-water (25:1:25, v/v) similarly by preparative HSCCC. The final structural identification was performed by MS, 1H-NMR, and 13C-NMR spectra.

引用

页码：1010 / 1022

页数：13

共 17 条

[1]

Application of high-speed counter-current chromatography for the isolation of 9'-cis-neoxanthin from fresh spinach [J].