Detection and Analysis of Low-Abundance Cell-Surface Biomarkers Using Enzymatic Amplification in Microfluidic Droplets

被引：118

作者：

Joensson, Haakan N. ^{[1
]}

Samuels, Michael L. ^{[2
]}

Brouzes, Eric R. ^{[2
]}

Medkova, Martina ^{[2
]}

Uhlen, Mathias ^{[3
]}

Link, Darren R. ^{[2
]}

Andersson-Svahn, Helene ^{[1
]}

机构：

[1] AlbaNova Univ Ctr, Royal Inst Technol, Dept Nano Biotechnol, S-10691 Stockholm, Sweden

[2] RainDance Technol, Lexington, MA 02421 USA

[3] AlbaNova Univ Ctr, Royal Inst Technol, Dept Prote, S-10691 Stockholm, Sweden

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 14期

基金：

瑞典研究理事会;

关键词：

droplets; enzymatic amplification; high-throughput screening; microfluidics; proteins; POLYMERASE-CHAIN-REACTION; PROTEIN EXPRESSION; SINGLE CELLS; ENCAPSULATION; GENE; CHIP;

D O I：

10.1002/anie.200804326

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Finding the few: Cell-surface proteins are useful disease biomarkers, but current highthroughput methods are limited to detecting cells expressing more than several hundred proteins. Enzymatic amplification in microfluidic droplets (see picture) is a high-throughput method for detection and analysis of cell-surface biomarkers expressed at very low levels on individual human cells. Droplet optical labels allow concurrent analysis of several samples. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2518 / 2521

页数：4

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