Isolation and purification of flavonoid glucosides from Radix Astragali by high-speed counter-current chromatography

被引：42

作者：

Xiao, Weihua ^{[1
]}

Han, Lujia ^{[1
]}

Shi, Bo ^{[2
]}

机构：

[1] China Agr Univ, Coll Engn, Beijing 100083, Peoples R China

[2] Chinese Acad Agr Sci, Feed Res Inst, Beijing 100081, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 8-9期

关键词：

High-speed counter-current chromatography; Radix Astragali; Isolation and purification; Calycosin-7-O-beta-D-glucoside-6 ''-O-acetate; MONGHOLICUS BGE. HSIAO; PREPARATIVE ISOLATION;

D O I：

10.1016/j.jchromb.2009.01.034

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

High-speed counter-current chromatography methods, combined with resin chromatography were applied to the separation and purification of flavonoid glycosides from the Chinese medicinal herb, Radix Astragali. Five flavonoid glycosides, namely calycosin-7-O-beta-D-glucoside, ononin, (6aR, 11aR)-9,10-dimethoxypterocarpan-3-O-beta-D-glucoside, (3R)-2'-hyclroxy-3',4'-dimethoxyisoflavan-7-O-beta-D-glucoside and calycosin-7-O-beta-D-glucoside-6 ''-O-acetate, were obtained. Among them, calycosin-7-O-beta-D-glucoside-6 ''-O-acetate was preparatively separated from Radix Astragali for the first time. Their structures were identified by ESI-MS, H-1 NMR, C-13 NMR, and 2D NMR. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：697 / 702

页数：6

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