Production of taxa-4(5),11(12)-diene by transgenic Physcomitrella patens

被引：65

作者：

Anterola, Aldwin ^{[1
]}

Shanle, Erin ^{[1
]}

Perroud, Pierre-Francois ^{[2
]}

Quatrano, Ralph ^{[2
]}

机构：

[1] So Illinois Univ, Dept Plant Biol, Carbondale, IL 62901 USA

[2] Washington Univ, Dept Biol, St Louis, MO 63130 USA

来源：

TRANSGENIC RESEARCH | 2009年 / 18卷 / 04期

关键词：

Taxa-4(5); 11(12)-diene; Taxadiene synthase; Physcomitrella patens; Taxol; Paclitaxel; Cancer; FUNCTIONAL EXPRESSION; TAXADIENE SYNTHASE; MOLECULAR-CLONING; ENDOPHYTIC FUNGI; TAXUS-BREVIFOLIA; TAXOL PRODUCTION; TAXANE CONTENT; BIOSYNTHESIS; STEP; YEW;

D O I：

10.1007/s11248-009-9252-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Taxadiene synthase gene from Taxus brevifolia was constitutively expressed in the moss Physcomitrella patens using a ubiquitin promoter to produce taxa-4(5),11(12)-diene, the precursor of the anticancer drug paclitaxel. In stable moss transformants, taxa-4(5),11(12)-diene was produced up to 0.05% fresh weight of tissue, without significantly affecting the amounts of the endogenous diterpenoids (ent-kaurene and 16-hydroxykaurane). Unlike higher plants that had been genetically modified to produce taxa-4(5),11(12)-diene, transgenic P. patens did not exhibit growth inhibition due to alteration of diterpenoid metabolic pools. Thus we propose that P. patens is a promising alternative host for the biotechnological production of paclitaxel and its precursors.

引用

页码：655 / 660

页数：6

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