Quantitative proteomics analysis of the secretory pathway

被引：394

作者：

Gilchrist, Annalyn

Au, Catherine E.

Hiding, Johan

Bell, Alexander W.

Fernandez-Rodriguez, Julia

Lesimple, Souad

Nagaya, Hisao

Roy, Line

Gosline, Sara J. C.

Hallett, Michael

Paiement, Jacques

Kearney, Robert E.

Nilsson, Tommy

Bergeron, John J. M.

机构：

[1] McGill Univ, Dept Anat & Cell Biol, Montreal, PQ H3A 2B2, Canada

[2] McGill Univ, McGill Ctr Bioinformat, Sch Comp Sci, Montreal, PQ H3A 2B4, Canada

[3] McGill Univ, Dept Biomed Engn, Montreal, PQ H3A 2B4, Canada

[4] Gothenburg Univ, Inst Biomed, Dept Med & Clin Genet, S-41390 Gothenburg, Sweden

[5] Gothenburg Univ, Sahlgrenska Acad, Proteom Ctr, S-41390 Gothenburg, Sweden

[6] Univ Montreal, Dept Pathol & Biol Cellulaire, Montreal, PQ H3C 3J7, Canada

来源：

CELL | 2006年 / 127卷 / 06期

基金：

加拿大健康研究院; 加拿大创新基金会;

关键词：

D O I：

10.1016/j.cell.2006.10.036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We report more than 1400 proteins of the secretory-pathway proteome and provide spatial information on the relative presence of each protein in the rough and smooth ER Golgi cisternae and Golgi-derived COPT vesicles. The data support a role for COPT vesicles in recycling and cisternal maturation, showing that Golgi-resident proteins are present at a higher concentration than secretory cargo. Of the 1400 proteins, 345 were identified as previously uncharacterized. Of these, 230 had their subcellular location deduced by proteomics. This study provides a comprehensive catalog of the ER and Golgi proteomes with insight into their identity and function.

引用

页码：1265 / 1281

页数：17

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