Magnesium uptake by CorA is essential for viability of the gastric pathogen Helicobacter pylori

被引：37

作者：

Pfeiffer, J ^{[1
]}

Guhl, J ^{[1
]}

Waidner, B ^{[1
]}

Kist, M ^{[1
]}

Bereswill, S ^{[1
]}

机构：

[1] Univ Hosp Freiburg, Dept Med Microbiol & Hyg, Inst Med Microbiol & Immunol, D-79104 Freiburg, Germany

来源：

INFECTION AND IMMUNITY | 2002年 / 70卷 / 07期

关键词：

D O I：

10.1128/IAI.70.7.3930-3934.2002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We show here that Mg2+ acquisition by CorA is essential for Helicobacter pylori in vitro. as corA mutants did not grow in media without Mg2+ supplementation. Complementation analysis performed with an Escherichia coli corA mutant revealed that H. pylori CorA transports nickel and cobalt in addition to Mg2+. However, Mg2+ is the dominant CorA substrate, as the corA mutation affected neither cobalt and nickel resistance nor nickel induction of urease in H. pylori. The drastic Mg2+ requirement (20 mM) of H. pylori corA mutants indicates that CorA plays a key role in the adaptation to the low-Mg2+ conditions predominant in the gastric environment.

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页码：3930 / 3934

页数：5

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