Effect of Soybean Volatile Compounds on Aspergillus flavus Growth and Aflatoxin Production

被引:52
作者
Cleveland, T. E. [1 ]
Carter-Wientjes, C. H. [1 ]
De Lucca, A. J. [1 ]
Boue, S. M. [1 ]
机构
[1] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70179 USA
关键词
aflatoxin; Aspergillus flavus; growth inhibition; soybean volatiles; MAIZE KERNELS; IN-VITRO; BOTRYTIS-CINEREA; LIPOXYGENASE; ALDEHYDES; DECAY; GERMINATION; RESISTANCE; VARIETIES; CONIDIA;
D O I
10.1111/j.1750-3841.2009.01078.x
中图分类号
TS2 [食品工业];
学科分类号
100403 [营养与食品卫生学];
摘要
Soybean homogenates produced volatile compounds upon exposure to lipase. These induced volatiles were identified by SPME. Seventeen volatile compounds identified by SPME were chosen for determination of their ability to inhibit Aspergillus flavus growth and aflatoxin B-1 (AFB1) production in a solid media assay. These volatiles included aldehydes, alcohols, ketones, and furans. Of the tested compounds, the aldehydes showed the greatest inhibition of fungal growth and AFB1 production. These compounds inhibited up to 100% of the observed growth and AFB1 production as compared to the controls. The greatest activity by the aldehydes to disrupt growth was ranked as follows: 2,4 hexadienal > benzaldehyde > 2-octenal > (E)-2-heptenal > octanal > (E)-2-hexenal > nonanal > hexanal. The greatest activity by the aldehydes to reduce AFB1 was ranked as follows: (E)-2-hexenal > 2,4 hexadienal > (E)-2-heptenal > hexanal > nonanal. (E)-2-hexenal and (E)-2-heptenal were tested further in an A. flavus-inoculated corn kernel assay. Both compounds prevented colonization by A. flavus and eliminated AFB1 production when exposed to compound volumes < 10 mu L as also shown in the solid media assay. The results suggest that soybeans react to lipase by production of potent antifungal volatiles.
引用
收藏
页码:H83 / H87
页数:5
相关论文
共 30 条
[1]
[Anonymous], [No title captured]
[2]
Fumigating 'Crimson Seedless' table grapes with (E)-2-hexenal reduces mold during long-term postharvest storage [J].
Archbold, DD ;
Hamilton-Kemp, TR ;
Clements, AM ;
Collins, RW .
HORTSCIENCE, 1999, 34 (04) :705-707
[3]
In vitro antifungal, and anti-elastase activity of some aliphatic aldehydes from Olea europaea L. fruit [J].
Battinelli, L. ;
Daniele, C. ;
Cristiani, M. ;
Bisignano, G. ;
Saija, A. ;
Mazzanti, G. .
PHYTOMEDICINE, 2006, 13 (08) :558-563
[4]
OCCURRENCE OF AFLATOXINS AND AFLATOXIN-PRODUCING STRAINS OF ASPERGILLUS SPP IN SOYBEANS [J].
BEAN, GA ;
SCHILLIN.JA ;
KLARMAN, WL .
APPLIED MICROBIOLOGY, 1972, 24 (03) :437-&
[5]
Effect of soybean lipoxygenase on volatile generation and inhibition of aspergillus flavus mycelial growth [J].
Boué, SA ;
Shih, BY ;
Carter-Wientjes, CH ;
Cleveland, TE .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (12) :4778-4783
[6]
SPREAD OF ASPERGILLUS-FLAVUS IN COTTON BOLLS, DECAY OF INTERCAPILLARY MEMBRANES, AND PRODUCTION OF FUNGAL PECTINASES [J].
BROWN, RL ;
CLEVELAND, TE ;
COTTY, PJ ;
MELLON, JE .
PHYTOPATHOLOGY, 1992, 82 (04) :462-467
[7]
LIVING MAIZE EMBRYO INFLUENCES ACCUMULATION OF AFLATOXIN IN MAIZE KERNELS [J].
BROWN, RL ;
COTTY, PJ ;
CLEVELAND, TE ;
WIDSTROM, NW .
JOURNAL OF FOOD PROTECTION, 1993, 56 (11) :967-971
[8]
BURROW GB, 1997, MOL PLANT MICROBE IN, V10, P380
[9]
Germination of Penicillium paneum conidia is regulated by 1-octen-3-ol, a volatile self-inhibitor [J].
Chitarra, GS ;
Abee, T ;
Rombouts, FM ;
Posthumus, MA ;
Dijksterhuis, J .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (05) :2823-2829
[10]
COTTY PJ, 1990, APPL ENVIRON MICROB, V61, P2372