Preparative and analytical chromatography of pegylated myelopoietin using monolithic media

被引：16

作者：

Hall, T ^{[1
]}

Wood, DC ^{[1
]}

Smith, CE ^{[1
]}

机构：

[1] Pfizer Inc, Chesterfield, MO 63017 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1041卷 / 1-2期

关键词：

monolithic media; myelopoietin; pegylated;

D O I：

10.1016/j.chroma.2004.04.037

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Monolithic media were compared with Q- and SP-Sepharose high performance chromatography for preparative purification and with Q-and SP-5PW chromatography for analysis of a pegylated form of myelopoietin (MPO), an engineered hematopoietic growth factor. The use of either monolithic or Sepharose based supports for preparative chromatography produced highly purified pegylated MPO with the monolithic media demonstrating peak resolution and repeatability at flow rates of 1 and 5 mVmin resulting in run times as much as five-fold shorter compared to Sepharose separations. The monolithic disks also resulted in 10-fold shorter run times for the analytical chromatography, however, their chromatographic profiles and peak symmetry were not as sharp compared to their Q-5PW and SP-5PW counterparts. (C) 2004 Elsevier B.V. All rights reserved.

引用

收藏

页码：87 / 93

页数：7

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