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Microsatellite primers for fungus-growing ants
被引:6
作者:
Villesen, P
Gertsch, PJ
Boomsma, JJ
[1
]
机构:
[1] Univ Copenhagen, Dept Populat Ecol, DK-2100 Copenhagen E, Denmark
[2] Aarhus Univ, Dept Ecol & Genet, DK-8000 Aarhus C, Denmark
[3] Uppsala Univ, Dept Conservat Biol & Genet, Evolutionary Biol Ctr, S-75007 Uppsala, Sweden
来源:
MOLECULAR ECOLOGY NOTES
|
2002年
/
2卷
/
03期
关键词:
attine ants;
Cyphomyrmex;
DNA microsatellites;
fungus-growing ants;
Trachymyrmex;
D O I:
10.1046/j.1471-8286.2002.00229.x
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
We isolated five polymorphic microsatellite loci from a library of two thousand recombinant clones of two fungus-growing ant species, Cyphomyrmex longiscapus and Trachymyrmex cf. zeteki. Amplification and heterozygosity were tested in five species of higher attine ants using both the newly developed primers and earlier published primers that were developed for fungus-growing ants. A total of 20 variable microsatellite loci, developed for six different species of fungus-growing ants, are now available for studying the population genetics and colony kin-structure of these ants.
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页码:320 / 322
页数:3
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