Cloning and expression of a putative transferrin cDNA of the spruce budworm, Choristoneura fumiferana

被引:24
作者
Ampasala, DR
Zheng, SC
Retnakaran, A
Krell, PJ
Arif, BM
Feng, QL
机构
[1] Canadian Forest Serv, Great Lakes Forestry Ctr, Nat Resources Canada, Sault Ste Marie, ON P6A 2E5, Canada
[2] Univ Guelph, Dept Microbiol, Guelph, ON N1G 2W1, Canada
关键词
transferrin; expression; iron-binding; antibiotics; antimicrobial protein; spruce budworm; Choristoneura jamiferana;
D O I
10.1016/j.ibmb.2004.03.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A spruce budworm (Choristoneura fumiferana) transferrin cDNA (CtTf) was isolated and cloned from a cDNA library that was constructed using mRNA from fifth to sixth instar larvae. CfTf cDNA encoded a predicted protein of 681 amino acids with a molecular mass of approximately 76 kDa. CtTf shared 72% and 74% identities at the amino acid level with transferrins of Manduca sexta and Bombyx mori, respecitvely. Like other transferrins, CtTf retains most of the N-terminal, iron-binding amino acid residues. Northern blot analyses indicated that CfTf mRNA was present at high levels after ecdysis, but that the expression level was low prior to ecdysis at the fourth-sixth instar stages. The highest level of CfTf expression was detected in the fat body. Relatively low levels of expression were detected in the epidermis and no expression was found in the midgut. Expression of CfTf mRNA could be induced by bacteria but not fungi. Expression of CfTf mRNA was suppressed by iron load. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:493 / 500
页数:8
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