Chiral resolution of cromakalim by HPLC on teicoplanin and teicoplanin aglycon chiral stationary phases

被引：17

作者：

Aboul-Enein, HY ^{[1
]}

Ali, I ^{[1
]}

机构：

[1] King Faisal Specialist Hosp & Res Ctr, Dept Biol & Med Res, Pharmaceut Anal Lab, MBC03, Riyadh 11211, Saudi Arabia

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2002年 / 25卷 / 13-15期

关键词：

cromakalim; stereoselective chromatography; teicoplanin; teicoplanin aglycon; macrocyclic glycopeptide antibiotics;

D O I：

10.1081/JLC-120014007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The chiral resolution of cromakalim. has been achieved on macrocyclic glycopeptide antibiotics columns, namely teicoplanin and teicoplanin aglycon known as Chirobiotic T and Chirobiotic TAG, respectively. The mobile phases used were hexane-ethanol (95 : 5, v/v) on Chirobiotic T and hexane-ethanol (75: 25, v/v) on Chirobiotic TAG columns, respectively, at a flow rate of 0.5 mL/min. The detection was carried out at 220 nm. The alpha values of the resolved enantiomers were 1.13 and 1.12 on Chirobiotic T and Chirobiotic TAG columns, respectively. The values of Rs were 1.27 and 1.25 on Chirobiotic T and Chirobiotic TAG columns, respectively. The detection of the enantiomers was better on Chirobiotic T column than on Chirobiotic TAG column.

引用

页码：2337 / 2344

页数：8

共 14 条

[1]

Aboul-Enein H.Y., 1997, IMPACT STEREOCHEMIST

[2] Macrocyclic antibiotics as effective chiral selectors for enantiomeric resolution by liquid chromatography and capillary electrophoresis [J].