Tissue-specific alternative RNA splicing of rat vesicle-associated membrane protein-1 (VAMP-1)

被引：14

作者：

Mandic, R

Trimble, WS

Lowe, AW

机构：

[1] STANFORD UNIV,SCH MED,DEPT MED,STANFORD,CA 94305

[2] STANFORD UNIV,SCH MED,CTR DIGEST DIS,STANFORD,CA 94305

[3] UNIV TORONTO,DEPT PHYSIOL,TORONTO,ON M5G 1X8,CANADA

[4] HOSP SICK CHILDREN,DIV CELL BIOL,TORONTO,ON M5G 1X8,CANADA

来源：

GENE | 1997年 / 199卷 / 1-2期

关键词：

cloning; gene; intron; isoform; secretion;

D O I：

10.1016/S0378-1119(97)00244-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The vesicle-associated membrane protein (VAMP) family is essential to vesicle-mediated protein transport. Three mammalian isoforms, VAMP-1, VAMP-2, and cellubrevin, play a role in protein transport to the plasma membrane. In this study, we describe a new rat VAMP-1 isoform produced by alternative pre-mRNA splicing. Only one VAMP-1 isoform dominates in each tissue. Analysis of the nucleotide sequence for the newly discovered isoform, VAMP-lb, reveals that its expression is determined by whether an intron is retained or removed. The predicted amino acid sequences for the VAMP-1 isoforms differ at the carboxyterminal end of the protein. A similar process has been described for VAMPs in Drosophila melanogaster and suggests a conserved function for the carboxy-terminal domain that can be modulated. (C) 1997 Elsevier Science B.V.

引用

页码：173 / 179

页数：7

共 31 条

[1]

ARCHER BT, 1990, J BIOL CHEM, V265, P17267

[2] LOCALIZATION OF SED5, A PUTATIVE VESICLE TARGETING MOLECULE, TO THE CIS-GOLGI NETWORK INVOLVES BOTH ITS TRANSMEMBRANE AND CYTOPLASMIC DOMAINS [J].