The effect of anti-inflammatory properties of ferritin light chain on lipopolysaccharide-induced inflammatory response in murine macrophages

被引：69

作者：

Fan, Yumei ^{[1
,2
,3
]}

Zhang, Jie ^{[1
,2
,3
]}

Cai, Linlin ^{[1
,2
,3
]}

Wang, Shengnan ^{[1
,2
,3
]}

Liu, Caizhi ^{[1
,2
,3
]}

Zhang, Yongze ^{[1
,2
,3
]}

You, Linhao ^{[1
,2
,3
]}

Fu, Yujian ^{[1
,2
,3
]}

Shi, Zhenhua ^{[1
,2
,3
]}

Yin, Zhimin ^{[4
]}

Luo, Lan ^{[5
]}

Chang, Yanzhong ^{[1
,2
,3
]}

Duan, Xianglin ^{[1
,2
,3
]}

机构：

[1] Hebei Normal Univ, Coll Life Sci, Lab Mol Iron Metab, Shijiazhuang 050024, Hebei Province, Peoples R China

[2] Hebei Normal Univ, Coll Life Sci, Key Lab Anim Physiol Biochem & Mol Biol Hebei Pro, Shijiazhuang 050024, Hebei Province, Peoples R China

[3] Hebei Normal Univ, Coll Life Sci, Minist Educ, Key Lab Mol & Cellular Biol, Shijiazhuang 050024, Hebei Province, Peoples R China

[4] Nanjing Normal Univ, Coll Life Sci, Jiangsu Prov Key Lab Mol & Med Biotechnol, Nanjing 210046, Jiangsu, Peoples R China

[5] Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Jiangsu, Peoples R China

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 2014年 / 1843卷 / 11期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

Ferritin light chain; Inflammatory response; LIP; ROS; MAPKs; NF-kappa B; NF-KAPPA-B; TUMOR-NECROSIS-FACTOR; NITRIC-OXIDE PRODUCTION; RAW; 264.7; MACROPHAGES; IRON REGULATORY PROTEIN-2; OXYGEN SPECIES FORMATION; OXIDATIVE STRESS; UP-REGULATION; LABILE IRON; LUNG INFLAMMATION;

D O I：

10.1016/j.bbamcr.2014.06.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Ferritin light chain (FTL) reduces the free iron concentration by forming ferritin complexes with ferritin heavy chain (FTH). Thus, FTL competes with the Fenton reaction by acting as an antioxidant. In the present study, we determined that FTL influences the lipopolysaccharide (LPS)-induced inflammatory response. FTL protein expression was regulated by LPS stimulation in RAW264.7 cells. To investigate the role of FTL in LPS-activated murine macrophages, we established stable FTL-expressing cells and used shRNA to silence FTL expression in RAW264.7 cells. Overexpression of FTL significantly decreased the LPS-induced production of tumor necrosis factor alpha (TNF-alpha), interleukin 1 beta (IL-1 beta), nitric oxide (NO) and prostaglandin E2 (PGE2). Additionally, overexpression of FTL decreased the LPS-induced increase of the intracellular labile iron pool (LIP) and reactive oxygen species (ROS). Moreover, FTL overexpression suppressed the LPS-induced activation of MAPKs and nuclear factor-kappa B (NF-kappa B). In contrast, knockdown of FTL by shRNA showed the reverse effects. Therefore, our results indicate that FTL plays an anti-inflammatory role in response to LPS in murine macrophages and may have therapeutic potential for treating inflammatory diseases. (C) 2014 Published by Elsevier B.V.

引用

页码：2775 / 2783

页数：9

共 55 条

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