Monoclonal antibody #5-2-26 recognizes the phosphatase-sensitive epitope of rabies virus nucleoprotein

被引：12

作者：

Kawai, A ^{[1
]}

Anzai, J ^{[1
]}

Honda, Y ^{[1
]}

Morimoto, K ^{[1
]}

Takeuchi, K ^{[1
]}

Kohno, T ^{[1
]}

Wakisaka, K ^{[1
]}

Goto, H ^{[1
]}

Minamoto, N ^{[1
]}

机构：

[1] GIFU UNIV,FAC AGR,DEPT VET PUBL HLTH,GIFU 50111,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1997年 / 41卷 / 01期

关键词：

monoclonal antibody; rabies virus nucleoprotein; phosphoprotein; phosphorylation; N gene expression;

D O I：

10.1111/j.1348-0421.1997.tb01170.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We prepared monoclonal antibodies (MAbs) against the rabies virus N protein, among which one antibody (MAb 5-2-26) was shown to lack reactivity with the phosphatase-treated N protein, The MAb was able to recognize the sodium dodecyl sulfate (SDS)-denatured N protein. The MAb did not recognize the N-protein analogues produced in Escherichia coli (E. coli), indicating that the N-gene products were not normally processed in E. coli after translation. On the other hand, the MAb reacted normally with N-gene products produced in COS-7 cells, but not with those produced in the presence of K-252a (a protein kinase inhibitor of a broad spectrum), The MAb displayed weak cross-reactivity with the Triton-insoluble network structures composed of several components, while another phosphoprotein (Mi) of the virus was not recognized at all, These results suggest that MAb 5-2-26 preferentially recognizes a phosphatase-sensitive linear epitope of N protein, which may enable further investigations to be conducted on the mechanism of N-protein phosphorylation and its role(s) in virus replication.

引用

页码：33 / 42

页数：10

共 32 条

[1]

Banerjee A K, 1990, Adv Virus Res, V38, P99

[2] GENE-EXPRESSION OF NONSEGMENTED NEGATIVE STRAND RNA VIRUSES [J].