Molecular cloning and characterization of an IgE-reactive protein from Anisakis simplex:: Ani s 1

被引：34

作者：

Arrieta, I

del Barrio, M

Vidarte, L

del Pozo, V

Pastor, C

Gonzalez-Cabrero, J

Cárdaba, B

Rojo, M

Mínguez, A

Cortegano, I

Gallardo, S

Aceituno, E

Palomino, P

Vivanco, F

Lahoz, C ^{[1
]}

机构：

[1] Fdn Jimenez Diaz, Dept Immunol, E-28040 Madrid, Spain

[2] Fdn Jimenez Diaz, Dept Res, E-28040 Madrid, Spain

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 2000年 / 107卷 / 02期

关键词：

helminth; parasite hypersensitivity; molecular cloning; mRNA; calcium-binding protein; EF-hand; Anisakis simplex; expression library;

D O I：

10.1016/S0166-6851(00)00192-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Ingestion of the parasitic nematode Anisakis,simplex in undercooked fish can cause severe allergic reactions in some individuals. Using pooled human sera from sensitized patients we have probed an expression library for A. simplex antigens. One positive clone was found to encode a full length 21 kDa protein with strong homology to nematode troponins. The recombinant protein was expressed as a GST-fusion protein and found by immunoblot analysis to react with sera from 20% of allergic patients. The presence of functional EF-hand Ca2+ binding motifs was demonstrated by gel-shift analysis. (C) 2000 Published by Elsevier Science B.V. All rights reserved.

引用

页码：263 / 268

页数：6

共 18 条

[1]

ARDUSOLOVERA DD, 1996, REV ESP ALERGOL INMU, V2, P280

[2]

RECURRENT ANAPHYLAXIS CAUSED BY ANISAKIS SIMPLEX PARASITIZING FISH [J].