Searching for quantitative entropy-enthalpy compensation among protein variants

被引：16

作者：

Beasley, JR ^{[1
]}

Doyle, DF ^{[1
]}

Chen, LX ^{[1
]}

Cohen, DS ^{[1
]}

Fine, BR ^{[1
]}

Pielak, GJ ^{[1
]}

机构：

[1] Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA

来源：

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS | 2002年 / 49卷 / 03期

关键词：

entropy-enthalpy compensation; protein thermodynamics;

D O I：

10.1002/prot.10189

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Entropy-enthalpy (SH) compensation occurs when a small change in OG is caused by large, and nearly compensatory, changes in OH and OS. It is considered a ubiquitous property of reactions in water. Because water is intimately involved in protein stability, SH compensation among protein variants, if it exists, could lead to important knowledge about protein-water interactions. In light of recent theoretical work on SH compensation, we gathered thermodynamic data for > 200 protein variants to seek evidence for the simplest quantitative model of SH compensation (i.e., The van't Hoff denaturation enthalpy divided by the van't Hoff denaturation entropy is a constant). We conclude that either the data are insufficient to support the idea that quantitative SH compensation is a general feature of variant proteins or that such compensation does not exist. This study reinforces the idea that OH-versus-OS plots should not be used to provide evidence for SH compensation. (C) 2002 Wiley-Liss, Inc.

引用

页码：398 / 402

页数：5

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