Antiviral effects of saikosaponins on human coronavirus 229E in vitro

被引:217
作者
Cheng, Pei-Win
Ng, Lean-Teik
Chiang, Lien-Chai
Lin, Chun-Ching
机构
[1] Kaohsiung Med Univ, Grad Inst Nat Prod, Kaohsiung 807, Taiwan
[2] Kaohsiung Med Univ, Dept Microbiol, Kaohsiung 807, Taiwan
[3] Tajen Univ, Dept Biotechnol, Pingtung, Taiwan
来源
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY | 2006年 / 33卷 / 07期
关键词
antiviral activity; saikosaponins; viral replication;
D O I
10.1111/j.1440-1681.2006.04415.x
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
1. Saikosaponins represent a group of oleanane derivatives, usually as glucosides, that are found in a number of plant families. Saikosaponins isolated from medicinal plants such as Bupleurum spp., Heteromorpha spp. and Scrophularia scorodonia have been reported to possess various biological activities, specifically antihepatitis, antinephritis, antihepatoma, anti-inflammation, immunomodulation and antibacterial effects. 2. The aim of the present study was to examine the anticoronaviral activity of saikosaponins (A, B-2, C and D) and their mode of action. Using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-5-[(phenylamino) carbonyl-2H-tetrazolium hydroxide] (XTT) assay, results showed that all saikosaponins tested demonstrated antiviral activity at concentrations of 0.25-25 mu mol/L, with the strongest activity being noted for saikosaponin B-2 (IC50 = 1.7 +/- 0.1 mu mol/L). Interestingly, both saikosaponins A (50% cellular cytotoxicity (CC50) concentration = 228.1 +/- 3.8 mu mol/L; selectivity index (SI) = 26.6) and B-2 (CC50 = 383.3 +/- 0.2 mu mol/L; SI = 221.9) exhibited no cytotoxic effects on target cells at concentrations that achieved antiviral activity. In the time-of-addition studies, saikosaponin B-2, at 6 mu mol/L, significantly inhibited human coronavirus 229E infection following its addition at various time pre-infection (-4 to -1 h), coinfection (0 h) and post-infection (1-4 h). Furthermore, saikosaponin B-2 also showed an inhibitory effect on viral attachment and penetration. 3. The present results indicate that saikosaponin B-2 has potent anticoronaviral activity and that its mode of action possibly involves interference in the early stage of viral replication, such as absorption and penetration of the virus.
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收藏
页码:612 / 616
页数:5
相关论文
共 24 条
[1]   SCH 43478 and analogs: in vitro activity and in vivo efficacy of novel agents for herpesvirus type 2 [J].
Albin, R ;
Chase, R ;
Risano, C ;
Lieberman, M ;
Ferrari, E ;
Skelton, A ;
Buontempo, P ;
Cox, S ;
DeMartino, J ;
WrightMinogue, J ;
JirauLucca, G ;
Kelly, J ;
Afonso, A ;
Kwong, AD ;
Rozhon, EJ ;
OConnell, JF .
ANTIVIRAL RESEARCH, 1997, 35 (03) :139-146
[2]   INVITRO ANTIVIRAL ACTIVITY OF A SAPONIN FROM ANAGALLIS-ARVENSIS, PRIMULACEAE, AGAINST HERPES-SIMPLEX VIRUS AND POLIOVIRUS [J].
AMOROS, M ;
FAUCONNIER, B ;
GIRRE, RL .
ANTIVIRAL RESEARCH, 1987, 8 (01) :13-25
[3]   ANTIVIRAL ACTIVITY OF GLYCYRRHIZIN AGAINST VARICELLA-ZOSTER VIRUS INVITRO [J].
BABA, M ;
SHIGETA, S .
ANTIVIRAL RESEARCH, 1987, 7 (02) :99-107
[4]   In vivo and in vitro antiinflammatory activity of saikosaponins [J].
Benito, PB ;
Martínez, MJA ;
Sen, AMS ;
Gómez, AS ;
Matellano, LF ;
Contreras, SS ;
Lanza, AMD .
LIFE SCIENCES, 1998, 63 (13) :1147-1156
[5]   Antiviral activity of seven iridoids, three saikosaponins and one phenylpropanoid glycoside extracted from Bupleurum rigidum and Scrophularia scorodonia [J].
Bermejo, P ;
Abad, MJ ;
Díaz, AM ;
Fernández, L ;
De Santos, J ;
Sanchez, S ;
Villaescusa, L ;
Carrasco, L ;
Irurzun, A .
PLANTA MEDICA, 2002, 68 (02) :106-110
[6]   Ethanol extract of Polygonum cuspidatum inhibits hepatitis B virus in a stable HBV-producing cell line [J].
Chang, JS ;
Liu, HW ;
Wang, KC ;
Chen, MC ;
Chiang, LC ;
Hua, YC ;
Lin, CC .
ANTIVIRAL RESEARCH, 2005, 66 (01) :29-34
[7]   Putranjivain A from Euphorbia jolkini inhibits both virus entry and late stage replication of herpes simplex virus type 2 in vitro [J].
Cheng, HY ;
Lin, TC ;
Yang, CM ;
Wang, KC ;
Lin, LT ;
Lin, CC .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2004, 53 (04) :577-583
[8]  
Chiang LC, 2003, PLANTA MED, V69, P705, DOI 10.1055/s-2003-42797
[9]   Antiviral activity of Plantago major extracts and related compounds in vitro [J].
Chiang, LC ;
Chiang, W ;
Chang, MY ;
Ng, LT ;
Lin, CC .
ANTIVIRAL RESEARCH, 2002, 55 (01) :53-62
[10]  
DAVID LE, 2005, ANTIVIR RES, V68, P124