Antigen binding characteristics of antibodies against hydroxyl radical modified thymidine monophosphate

被引：10

作者：

Ahmad, R ^{[1
]}

Alam, K ^{[1
]}

Ali, R ^{[1
]}

机构：

[1] Aligarh Muslim Univ, JN Med Coll, Fac Med, Dept Biochem, Aligarh 202002, Uttar Pradesh, India

来源：

IMMUNOLOGY LETTERS | 2000年 / 71卷 / 02期

关键词：

hydroxyl radical; TMP-BSA and ROS-TMP-BSA conjugates; anti-DNA antibodies; SLE;

D O I：

10.1016/S0165-2478(99)00177-7

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Reactive oxygen species (ROS) are formed in living organisms during normal metabolic reactions as well as under different environmental stresses. In this study, thymidine monophosphate (TMP) was exposed to hydroxyl radical (OH) and challenged in rabbits. TMP and OH-modified TMP were found to be nonimmunogenic. The TMP was linked to bovine serum albumin (BSA) by carbodiimide reaction, and then modified with the OH. The neoantigens, TMP-BSA, and ROS-TMP-BSA conjugates induced highly specific antibodies against immunogens. Induced antibodies exhibited appreciable cross-reactivity with various polynucleotides and nucleic acids. In this respect, the induced antibodies resembled the diverse antigen-binding characteristics of naturally occurring systemic lupus erythematosus (SLE) anti-DNA autoantibodies. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：111 / 115

页数：5

共 22 条

[1] THE EFFECT OF HYDROXYL RADICAL ON THE ANTIGENICITY OF NATIVE DNA [J].