Substrate-dependent production and some properties of a thermostable, α-galactosidase from Rhodothermus marinus

被引：19

作者：

Blücher, A

Karlsson, EN

Holst, O

机构：

[1] Kalmar Univ, Dept Nat Sci, S-39129 Kalmar, Sweden

[2] Lund Univ, Ctr Chem & Chem Engn, S-22100 Lund, Sweden

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 08期

关键词：

galactosides; glycosyl hydrolase; thermophilic;

D O I：

10.1023/A:1005627501609

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

alpha-Galactosidase activity in Rhodothermus marinus is dependent on the composition of the growth media. A maximum of 46 mu U g(-1) cell dry weight was obtained using minimal medium with galactooligo- or polysaccharides as single carbon source. An enzyme hydrolysing both high and low molecular weight galacto-saccharides was partly purified from the cell fractions. The molecular weight was 200 kDa (native) and 50 kDa (monomer). It was optimally active at 85 degrees C, with a half-life of 2 h at 75 degrees C, and had a broad pH range (4-8).

引用

页码：663 / 669

页数：7

共 23 条

[1]

ALFREDSSON GA, 1988, J GEN MICROBIOL, V134, P299

[2] DEVELOPMENT OF A BIOTECHNOLOGICAL PROCESS FOR THE MODIFICATION OF GALACTOMANNAN POLYMERS WITH PLANT ALPHA-GALACTOSIDASE [J].