Anti-tumor and immunomodulating effects of Pleurotus ostreatus mycelia-derived proteoglycans

被引：325

作者：

Sarangi, Itisam ^{[1
]}

Ghosh, Dipanjan ^{[1
]}

Bhutia, Sujit Kumar ^{[1
]}

Mallick, Sanjaya Kumar ^{[1
]}

Maiti, Tapas K. ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2006年 / 6卷 / 08期

关键词：

mushroom; Pleurotus ostreatus; mycelia; proteoglycan; immunomodulatory; anti-cancer;

D O I：

10.1016/j.intimp.2006.04.002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

Pleurotus ostreatits is one of the widely cultivated edible mushrooms. Water-soluble proteoglycan fractions from P. ostreatus mycelia were purified by alcohol-precipitation, ion exchange and followed by gel permeation (Sephadex G-100) chromatography. Three neutral fractions were found, which had polysaccharide to protein ratios 14.2, 26.4 and 18.3, respectively. These fractions were tested for in vitro and in vivo immunomodulatory and anticancer effects on Sarcoma-180-bearing mouse model. In vivo injection of proteoglycans to Sarcoma-180-bearing mice decreased the number of tumor cells and cell cycle analysis showed that most of the cells were found to be arrested in pre-G(0)/G(1) phase of cell cycle. All of the three proteoglycans elevated mouse natural killer (NK) cell cytotoxicity and stimulated macrophages to produce nitric oxide. The Fourier transform infra red (FTIR) spectra suggested the presence of beta-glycosidic bond in all the fractions. Fraction I strongly interacted with glucose/mannose-specific lectin Concanavalin A (ConA), indicating the presence of large number of terminal sugar with glucose/mannose. Thus, the three neutral proteoglycans derived from the mushroom (P. ostreatus) mycelia could be used as immunomodulators and anti cancer agents. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：1287 / 1297

页数：11

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