New chlorogenin hexasaccharide isolated from Agave fourcroydes with cytotoxic and cell cycle inhibitory activities

被引：45

作者：

Ohtsuki, T

Koyano, T

Kowithayakorn, T

Sakai, S

Kawahara, N

Goda, Y

Yamaguchi, N

Ishibashi, M

机构：

[1] Chiba Univ, Grad Sch Pharmaceut Sci, Inage Ku, Chiba 2638522, Japan

[2] Temko Corp, Nakano Ku, Tokyo 1640012, Japan

[3] Khon Kaen Univ, Fac Agr, Dept Hort, Khon Kaen 40002, Thailand

[4] Natl Inst Hlth Sci, Div Pharmacognosy Phytochem & Narcot, Setagaya Ku, Tokyo 1588501, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2004年 / 12卷 / 14期

关键词：

Agavaceae; Agave fourcroydes; steroidal saponin; cytotoxicity; cell cycle;

D O I：

10.1016/j.bmc.2004.05.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new chlorogenin hexasaccharide (1) was isolated from leaves of Agave fourcroydes (Agavaceae). The structure of the new saponin was elucidated as chlorogenin 3-O-[alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->3)-{beta-D-glucopyranosyl-(1-->3)-beta-D-glucopyranosyl-(1-->2)}-beta-D-glucopyranosyl-(1-->4)-beta-D-galactopyranoside] (1) by spectroscopic analysis and the result of acidic hydrolysis. The new saponin (1) as well as known hexasaccharides (3 and 5) isolated here showed cytotoxicity against HeLa cells, and I exhibited a cell cycle inhibitory effect at the G2/M stage at the concentration of 7.5 and 10 mug/mL. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：3841 / 3845

页数：5

共 18 条

[1] C-13 NMR SPECTRAL INVESTIGATIONS .10. C-13 NMR-SPECTROSCOPY OF STEROIDAL SAPOGENINS AND STEROIDAL SAPONINS [J].