FilmArray® respiratory panel performance in respiratory samples from neonatal care units

被引：24

作者：

Piralla, Antonio ^{[1
]}

Lunghi, Giovanna ^{[2
]}

Percivalle, Elena ^{[1
]}

Vigano, Cristina ^{[2
]}

Nasta, Teresa ^{[2
]}

Pugni, Lorenza ^{[3
]}

Mosca, Fabio ^{[3
]}

Stronati, Mauro ^{[4
]}

Torresani, Erminio ^{[2
]}

Baldanti, Fausto ^{[1
]}

机构：

[1] Fdn IRCCS Policlin San Matteo, Microbiol & Virol Dept, Mol Virol Unit, I-27100 Pavia, Italy

[2] Fdn Ca Granda Osped Maggiore, Virol Unit, Milan, Italy

[3] Fdn Ca Granda Osped Maggiore, Neonatol & Intens Neonatal Care Unit, Milan, Italy

[4] Fdn IRCCS Policlin San Matteo, Neonatal Dept, I-27100 Pavia, Italy

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2014年 / 79卷 / 02期

关键词：

Respiratory viruses; Multiple PCR tests; FilmArray; TIME RT-PCR; HUMAN CORONAVIRUS; XTAG RVP; CHILDREN; ASSAY; PATHOGENS; VIRUSES; INFECTIONS; SPECIMENS; OUTBREAK;

D O I：

10.1016/j.diagmicrobio.2014.02.010

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

FilmArray Respiratory Panel (RP) (Idaho Technology, Inc., Salt Lake City, UT, USA) performance was retrospectively evaluated in respiratory samples collected from neonates in 2 reference neonatology units. Using the FilmArray RP assay, 121/152 (79.6%) samples were positive for at least 1 respiratory virus, while 31/ 152 (20.4%) were negative. FilmArray RP results were concordant in 68/72 (94.4%) respiratory samples tested with laboratory-developed real-time PCR assays, while in 4/72 (5.6%) samples, the FilmArray RP assay detected an additional virus (2 human rhinovirus/enterovirus and 2 bocavirus). In addition, FilmArray RP results for 70 of 80 (87.5%) respiratory samples tested were concordant with the Seegene Seeplex RV15 detection assay (Seegene, Inc., Seoul, South Korea), while 10/80 (12.5%) were discordant. The advantages of the FilmArray RP are the rapid detection of respiratory viruses (1 hour), the wide number of pathogens detectable in a single assay, and the reduced hands-on time. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：183 / 186

页数：4

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