Innovation - Seventeen-colour flow cytometry: unravelling the immune system

被引：750

作者：

Perfetto, SP ^{[1
]}

Chattopadhyay, PK ^{[1
]}

Roederer, M ^{[1
]}

机构：

[1] NIAID, ImmunoTechnol Sect, Vaccine Res Ctr, NIH, Bethesda, MD 20892 USA

来源：

NATURE REVIEWS IMMUNOLOGY | 2004年 / 4卷 / 08期

关键词：

D O I：

10.1038/nri1416

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The increasing need for polychromatic approaches to flow cytometry, coupled with rapid technological advances, has pushed the frontiers of flow cytometry beyond 12-colour systems. Recent breakthroughs have allowed the design and implementation of instruments that measure 19 parameters ( 17 fluorescent colours and 2 physical parameters). This article describes the instrumentation and considers the reagents, analysis and applications for this powerful, new extension of flow-cytometric technology.

引用

页码：648 / U5

页数：9

共 33 条

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