Fimbrial-dependent mating in Microbotryum violaceum involves a mannose-lectin interaction

被引:7
作者
Castle, AJ
Stocco, N
Boulianne, R
机构
[1] Department of Biological Sciences, Brock University, St. Catharines
关键词
mating; Microbotryum violaceum; lectin; fimbriae;
D O I
10.1139/m96-063
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fimbriae of the anther smut fungus, Microbotryum violaceum are polymers of six 74-kDa glycoprotein isoforms. Digestion of fimbrial monomers with alpha-mannosidase yielded two polypeptides with masses of 70 and 48 kDa. The 70-kDa polypeptide is probably a product of incomplete digestion and the 48-kDa polypeptide is the aglycone. Thus, most of the carbohydrate component of fimbrial protein is mannose. Previous observations have suggested that fimbriae are necessary for mating in M. violaceum. Further evidence for this role was obtained in the present study by showing that mating is inhibited by an anti-fimbrial protein antiserum, by mannose and related sugars glucose and arabinose, and by the lectin concanavalin A. Since inhibition was not complete, however, two mechanisms for adhesion between compatible cells were proposed, one fimbrial dependent and one independent. Lastly, fimbrial protein from a(1) but not a(2) mating types bound to a mannose-agarose column, suggesting a lectin-like capability. The fimbrial dependent mechanism of cell-to-cell adhesion may involve binding of the mannose residues of the fimbriae of a(2) cells by the fimbriae of a(1) cells.
引用
收藏
页码:461 / 466
页数:6
相关论文
共 21 条
[1]  
ALMAHMOOD S, 1988, J BIOL CHEM, V263, P3930
[2]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3]  
CALDERONE RA, 1991, MICROBIOL REV, V55, P1
[4]   POSTTRANSLATIONAL MODIFICATION OF FIMBRIAL PROTEIN FROM USTILAGO-VIOLACEA [J].
CASTLE, AJ ;
BOULIANNE, R ;
XU, JH ;
DAY, AW .
CANADIAN JOURNAL OF MICROBIOLOGY, 1992, 38 (11) :1144-1149
[5]   A GLYCOSYLATION PATTERN THAT IS UNIQUE TO FIMBRIAE FROM THE TAXON MICROBOTRYALES [J].
CELERIN, M ;
DAY, AW ;
CASTLE, AJ ;
LAUDENBACH, DE .
CANADIAN JOURNAL OF MICROBIOLOGY, 1995, 41 (06) :452-460
[6]   EVIDENCE THAT FIMBRIAE OF THE SMUT FUNGUS MICROBOTRYUM-VIOLACEUM CONTAIN RNA [J].
CELERIN, M ;
LAUDENBACH, DE ;
BANCROFT, JB ;
DAY, AW .
MICROBIOLOGY-SGM, 1994, 140 :2699-2704
[7]   FUNGAL FIMBRIAE .2. THEIR ROLE IN CONJUGATION IN USTILAGO-VIOLACEA [J].
DAY, AW ;
POON, NH .
CANADIAN JOURNAL OF MICROBIOLOGY, 1975, 21 (04) :547-557
[8]   PRODUCTION AND CHARACTERISTICS OF DIPLOIDS IN USTILAGO VIOLACEA [J].
DAY, AW ;
JONES, JK .
GENETICS RESEARCH, 1968, 11 (01) :63-+
[9]   COMMUNICATION THROUGH FIMBRIAE DURING CONJUGATION IN A FUNGUS [J].
DAY, AW .
NATURE, 1976, 262 (5569) :583-584
[10]   SOMATIC NUCLEAR DIVISION IN SPORIDIA OF USTILAGO-VIOLACEA .1. ACETIC ORCEIN STAINING [J].
DAY, AW ;
JONES, JK .
CANADIAN JOURNAL OF MICROBIOLOGY, 1972, 18 (05) :663-+