A simple method for monitoring chromatography column eluates for laccase activity during enzyme purification

被引：14

作者：

Dekker, RFH ^{[1
]}

Ling, KY ^{[1
]}

Barbosa, AM ^{[1
]}

机构：

[1] Murdoch Univ, Div Sci, Murdoch, WA 6150, Australia

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 02期

关键词：

ABTS; Botryosphaeria; chromogenic substrates; laccases; zymogram method;

D O I：

10.1023/A:1005680027798

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A simple and economical method is described that allows rapid detection of laccase activity in chromatography column fractions during enzyme purification. Aliquots of column eluants are applied to filter paper coated with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) containing a numbered grid, and incubated at ambient temperature for 20 min. Indications of enzyme activity are simply observed by a colour change. This method avoids having to manually assay each fraction of a chromatographic run for enzyme activity.

引用

页码：105 / 108

页数：4

共 8 条

[1] Veratryl alcohol as an inducer of laccase by an ascomycete, Botryosphaeria sp, when screened on the polymeric dye Poly R-478 [J].