Intrachromosomal recombination between attP regions as a tool to remove selectable marker genes from tobacco transgenes

被引:113
作者
Zubko, E [1 ]
Scutt, C [1 ]
Meyer, P [1 ]
机构
[1] Univ Leeds, Fac Biol Sci, Leeds Inst Plant Biotechnol & Agr, Leeds LS2 9JT, W Yorkshire, England
关键词
resistance marker; homologous recombination; transgenic plants;
D O I
10.1038/74515
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Recombinant genes conferring resistance to antibiotics or herbicides are widely used as selectable markers in plant transformation. Once transgenic material has been selected, the marker gene is dispensable. We report a novel strategy to remove undesirable parts of a transgene after integration into the tobacco genome, This approach is based on the transfer of a vector containing a NPTII gene flanked by two 352 bp attachment P (attP) regions of bacteriophage lambda, and the identification of somatic tissue with deletion events following intrachromosomal recombination between the attP regions. This system was used to delete a 5.9 kb region from a recombinant vector that had been inserted into two different genomic regions. As the attP system does not require the expression of helper proteins to induce deletion events, or a genetic segregation step to remove recombinase genes, it should provide a useful tool to remove undesirable transgene regions, especially in vegetatively propagated species.
引用
收藏
页码:442 / 445
页数:4
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