Microfluidic device reads up to four consecutive base pairs in DNA sequencing-by-synthesis

被引：64

作者：

Kartalov, EP ^{[1
]}

Quake, SR ^{[1
]}

机构：

[1] CALTECH, Dept Appl Phys, Pasadena, CA 91125 USA

来源：

NUCLEIC ACIDS RESEARCH | 2004年 / 32卷 / 09期

关键词：

D O I：

10.1093/nar/gkh613

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed the first fully integrated microfluidic system for DNA sequencing-by-synthesis. Using this chip and fluorescence detection, we have reliably sequenced up to 4 consecutive bps. The described sequencer can be integrated with other microfluidic components on the same chip to produce true lab-on-a-chip technology. The surface chemistry that was designed to anchor the DNA to elastomeric microchannels is useful in a broad range of studies and applications.

引用

收藏

页码：2873 / 2879

页数：7

相关论文

共 38 条

[1] Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules [J].

Akeson, M ;

Branton, D ;

Kasianowicz, JJ ;

Brandin, E ;

Deamer, DW .

BIOPHYSICAL JOURNAL, 1999, 77 (06) :3227-3233

[2] Photocleavage of a 2-nitrobenzyl linker bridging a fluorophore to the 5' end of DNA [J].

Bai, XP ;

Li, ZM ;

Jockusch, S ;

Turro, NJ ;

Ju, JY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (02) :409-413

[3] Sequence information can be obtained from single DNA molecules [J].

Braslavsky, I ;

Hebert, B ;

Kartalov, E ;

Quake, SR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (07) :3960-3964

[4] Nanopores and nucleic acids: prospects for ultrarapid sequencing [J].

Deamer, DW ;

Akeson, M .

TRENDS IN BIOTECHNOLOGY, 2000, 18 (04) :147-151

[5]

DRMANAC R, 1989, GENOMICS, V4, P114

[6] DNA sequencing using biotinylated dideoxynucleotides and mass spectrometry [J].

Edwards, JR ;

Itagaki, Y ;

Ju, JY .

NUCLEIC ACIDS RESEARCH, 2001, 29 (21) :art. no.-e104

[7]

Ehn M, 2002, ELECTROPHORESIS, V23, P3289, DOI 10.1002/1522-2683(200210)23:19<3289::AID-ELPS3289>3.0.CO

[8]

2-#

[9] Sequencing exons 5 to 8 of the p53 gene by MALDI-TOF mass spectrometry [J].

Fu, DJ ;

Tang, K ;

Braun, A ;

Reuter, D ;

Darnhofer-Demar, B ;

Little, DP ;

O'Donnell, MJ ;

Cantor, CR ;

Köster, H .

NATURE BIOTECHNOLOGY, 1998, 16 (04) :381-384

[10] A robust and scalable microfluidic metering method that allows protein crystal growth by free interface diffusion [J].

Hansen, CL ;

Skordalakes, E ;

Berger, JM ;

Quake, SR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (26) :16531-16536

← 1 2 3 4 →