Genetic synthesis and characterization of pH- and temperature-sensitive silk-elastinlike protein block copolymers

被引:66
作者
Nagarsekar, A
Crissman, J
Crissman, M
Ferrari, F
Cappello, J
Ghandehari, H [1 ]
机构
[1] Univ Maryland, Sch Pharm, Dept Pharmaceut Sci, Baltimore, MD 21201 USA
[2] Prot Polymer Technol Inc, San Diego, CA 92121 USA
来源
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH | 2002年 / 62卷 / 02期
关键词
drug delivery; genetic engineering; protein polymers; stimuli sensitive; silk-elastinlike protein;
D O I
10.1002/jbm.10272
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The purpose of this work was to synthesize and characterize a pH- and temperature-sensitive block copolymer containing repeating sequences from silk (Gly-Ala-Gly-Ala-Gly-Ser) and elastin (Gly-Val-Gly-Val-Pro) protein. The monomer contained one repeat of silk and eight repeat units of elastin, with the first valine in one of the elastin repeats being replaced by glutamic acid. The copolymer was synthesized using genetic engineering techniques. The sensitivity of the copolymer to pH and temperature was examined at various polymer concentrations and ionic strengths. Turbidity measurements were carried out over a temperature range of 20 to 100degreesC at various pH, concentration, and ionic strength values. The introduction of an ionizable residue (glutamic acid) rendered the copolymer sensitive to changes in pH. The transition termperature (T-t), the temperature at which the polymer became insoluble upon increase in temperature, was modulated by changing the pH. In general, the T-t value, was found: (1) to increase with an increase in pH, (2) to decrease with increasing ionic strength, and (3) to decrease with increasing concentration. Results of these studies suggest that by strategic placement of charged amino acids in genetically engineered silk-elastinlike protein block copolymers it is possible to precisely control sensitivity to stimuli such as pH and temperature. (C) 2002 Wiley Periodicals, Inc.
引用
收藏
页码:195 / 203
页数:9
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