Real-Time Trafficking of PEGylated Liposomes in the Rodent Focal Brain Ischemia Analyzed by Positron Emission Tomography

被引：32

作者：

Fukuta, Tatsuya ^{[1
]}

Ishii, Takayuki ^{[1
]}

Asai, Tomohiro ^{[1
]}

Nakamura, Genki ^{[1
]}

Takeuchi, Yoshihito ^{[1
]}

Sato, Akihiko ^{[1
]}

Agato, Yurika ^{[1
]}

Shimizu, Kosuke ^{[1
]}

Akai, Shuji ^{[2
]}

Fukumoto, Dai ^{[3
]}

Harada, Norihiro ^{[3
]}

Tsukada, Hideo ^{[3
]}

Kawaguchi, Akira T. ^{[4
]}

Oku, Naoto ^{[1
]}

机构：

[1] Univ Shizuoka, Dept Med Biochem, Sch Pharmaceut Sci, Shizuoka 4228526, Japan

[2] Osaka Univ, Dept Synthet Med Chem, Grad Sch Pharmaceut Sci, Osaka, Japan

[3] Hamamatsu Photon KK, Cent Res Lab, Hamamatsu, Shizuoka, Japan

[4] Tokai Univ, Sch Med, Isehara, Kanagawa 25911, Japan

来源：

ARTIFICIAL ORGANS | 2014年 / 38卷 / 08期

基金：

日本学术振兴会;

关键词：

Liposome; Positron emission tomography; Ischemic stroke; Drug delivery system; Permanent middle cerebral artery occlusion; ASIALO-ERYTHROPOIETIN; STROKE; PENUMBRA; INJURY;

D O I：

10.1111/aor.12350

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

A liposomal drug delivery system was previously applied to ischemic brain model rats for the treatment of brain ischemia, and we observed that 100-nm-sized liposomes could extravasate and accumulate in the ischemic brain region even when cerebral blood flow was markedly reduced in permanent middle cerebral artery occlusion (p-MCAO) model rats. In the present study, we investigated the real-time cerebral distribution of polyethylene glycol (PEG)-modified liposomes (PEG-liposomes) labeled with 1-[F-18]fluoro-3,6-dioxatetracosane in p-MCAO rats by positron emission tomography (PET). [F-18]-Labeled PEG-liposomes were intravenously injected into p-MCAO rats 1 h after the onset of occlusion, and then a PET scan was performed for 2 h. The PET scan showed that the signal intensity of [F-18] gradually increased in the ischemic region despite the drastic reduction in cerebral perfusion, suggesting that PEG-liposomes had accumulated in and around the ischemic region. Therefore, drug delivery to the ischemic region by use of liposomes would be possible under ischemic conditions, and a liposomal drug delivery system could be a promising strategy for protecting the ischemic brain from damage before recovery from ischemia.

引用

页码：662 / 666

页数：5

共 15 条

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Recombinant Tissue-Type Plasminogen Activator Use for Ischemic Stroke in the United States A Doubling of Treatment Rates Over the Course of 5 Years [J].