Mediated amperometric glucose sensor modified by the sol-gel method

An amperometric mediated glucose sensor has been developed via the sol-gel technique in a new 'sandwich' configuration: sol-gel-ferrocene:glucose oxidase (GOx):sol-gel using a carbon paste electrode as the primary electrode. The process parameters for the fabrication of a glucose sensor based on tetramethoxysilane (TMOS) sol-gel thin films utilizing ferrocene as a mediator have been optimized. In the sol-gel matrix, the results demonstrate that GOx still retains its activity, and ferrocene is efficient at shuttling the electrons between the enzyme redox centre and the electrode. Cyclic voltammetric and amperometric measurements have been used to study the response of the glucose sensor, which has a fast response and good reproducibility. The effect of oxygen on the sensor response has been investigated. The selectivity, lifetime and fabrication reproducibility of the sensor have also been evaluated. (C) 1997 Elsevier Science S.A.